ArticleMethods in enzymology2016
Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes.
Article in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Deciphering the molecular mechanisms of BPTF interactions with nucleosomes via molecular simulations.Biophysical journal · 2025Article
- Structural and thermodynamic impact of oncogenic mutations on the nucleosome core particle.Biophysical journal · 2025Article
- Deciphering the Molecular Mechanisms of BPTF Interactions with Nucleosomes via Molecular Simulations.bioRxiv : the preprint server for biology · 2025Article
- Basic helix-loop-helix pioneer factors interact with the histone octamer to invade nucleosomes and generate nucleosome-depleted regions.Molecular cell · 2023Article
- Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination.Journal of visualized experiments : JoVE · 2022Article
- Emerging Contributions of Solid-State NMR Spectroscopy to Chromatin Structural Biology.Frontiers in molecular biosciences · 2021Review
- Reconstitution and Purification of Nucleosomes with Recombinant Histones and Purified DNA.Current protocols in molecular biology · 2020Article
- Structural Basis of H2B Ubiquitination-Dependent H3K4 Methylation by COMPASS.Molecular cell · 2019Article
- Topology and enzymatic properties of a canonical Polycomb repressive complex 1 isoform.FEBS letters · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Structural studies of chromatin complexes composed of chromatin factors or enzymes bound to the nucleosome have been constrained by the ability to produce high-quality complexes in the amounts appropriate for biophysical studies and by the difficulty of crystallizing these complexes. We describe here procedures and approaches to prepare chromatin complexes, to crystallize chromatin complexes, and to improve diffraction properties through postcrystallization soaks. Special attention is paid to evaluating the quality of the purified chromatin complexes as well as assessing the presence of the chromatin protein or enzyme in crystals. The methods described for preparing and purifying chromatin complexes should be applicable to biochemical, biophysical, and other structural approaches including cryoelectron microscopy.
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