ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2016
Toxoplasma gondii Infection Promotes Epithelial Barrier Dysfunction of Caco-2 Cells.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Integrated Proteomic Profiling Reveals Dynamic Remodeling of the Intestinal Proteome inBiology · 2026Article
- IFN-γ protects the human blood-brain barrier from Toxoplasma gondii-mediated dysfunction via CCL2 suppression.Journal of neuroinflammation · 2026Article
- The gut metabolite arachidonic acid alleviates intestinal injury induced by a Toxoplasma gondii strain isolated from a wild rodent.Parasites & vectors · 2026Article
- Exploiting the cardioprotective potential of metformin against cardiotoxic agents.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Comprehensive analysis of Toxoplasma gondii migration routes and tissue dissemination in the host.PLoS neglected tropical diseases · 2025Review
- Modeling Toxoplasma gondii-gut early interactions using a human microphysiological system.PLoS neglected tropical diseases · 2025Article
- Article
- Trichinella spiralis cathepsin L damages the tight junctions of intestinal epithelial cells and mediates larval invasion.PLoS neglected tropical diseases · 2023Article
- Preventing occludin tight-junction disruption via inhibition of microRNA-193b-5p attenuates viral load and influenza-induced lung injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- The gut microbiota contributes to changes in the host immune response induced by Trichinella spiralis.PLoS neglected tropical diseases · 2023Article
- ExploringFrontiers in cellular and infection microbiology · 2023Review
- Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration.Scientific reports · 2022Article
- Association of Cardiotoxicity With Doxorubicin and Trastuzumab: A Double-Edged Sword in Chemotherapy.Cureus · 2021Review
- Early Immune Initiation by Porcine Cells followingMicroorganisms · 2021Article
- Disruption of Epithelial Barrier of Caco-2 Cell Monolayers by Excretory Secretory Products ofFrontiers in microbiology · 2021Article
- Structural, Functional, and Metabolic Alterations in Human Cerebrovascular Endothelial Cells duringMicroorganisms · 2020Article
- Harmonization of Protocols for Multi-Species Organoid Platforms to Study the Intestinal Biology ofFrontiers in cellular and infection microbiology · 2020Article
- From Initiators to Effectors: Roadmap Through the Intestine During Encounter ofFrontiers in cellular and infection microbiology · 2020Review
- Tryptophan-kynurenine pathway attenuates β-catenin-dependent pro-parasitic role of STING-TICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection.Cell death & disease · 2019Article
- From Entry to Early Dissemination-Frontiers in cellular and infection microbiology · 2019Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After oral infection, Toxoplasma gondii invades intestinal cells, induces breakdown of intestinal physiology and barrier functions, and causes intestinal pathology in some animal species. Although parasites' invasion into host cells is a known phenomenon, the effects of T. gondii infection in the intestinal barrier are still not well established. To evaluate morphological and physiological modifications on the colorectal adenocarcinoma-derived Caco-2 cell line during T. gondii infection, microvilli, tight junction integrity, and transepithelial electrical resistance (TEER) were investigated under infection. It was observed that the dextran uptake (endocytosis) and distribution were smaller in infected than in noninfected Caco-2 cells. The infection leads to the partial loss of microvilli at the cell surface. Claudin-1, zonula occludens-1 (ZO-1), and occludin expressions were colocalized by immunofluorescence and presented discontinuous net patterns in infected cells. Immunoblotting analysis at 24 hr postinfection revealed decreasing expression of occludin and ZO-1 proteins, whereas claudin-1 presented similar expression level compared with noninfected cells. T. gondii decreased TEER in Caco-2 cells 24 hr after infection. Our results suggest that T. gondii infection may lead to the loss of integrity of intestinal mucosa, resulting in impaired barrier function.
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