ArticleJournal of industrial microbiology & biotechnology2016
Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Development of a lysine biosensor for the dynamic regulation of cadaverine biosynthesis in E. coli.Microbial cell factories · 2025Article
- Medical Potential of Insect Symbionts.Insects · 2025Review
- High Throughput Screening of Transcription Factor LysG for Constructing a Better Lysine Biosensor.Biosensors · 2024Article
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- OptimizingFrontiers in microbiology · 2024Review
- Genetically Encoded Biosensor Engineering for Application in Directed Evolution.Journal of microbiology and biotechnology · 2023Review
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- A unified design allows fine-tuning of biosensor parameters and application across bacterial species.Metabolic engineering communications · 2020Article
- Discovery and identification of medium-chain fatty acid responsive promoters inEngineering in life sciences · 2020Article
- Transcription Factor Engineering for High-Throughput Strain Evolution and Organic Acid Bioproduction: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Metabolic engineering of glucose uptake systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production.Journal of industrial microbiology & biotechnology · 2019Article
- GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli.Microbial cell factories · 2019Article
- High-yield production of L-serine from glycerol by engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Article
- Deep scanning lysine metabolism inMolecular systems biology · 2018Article
- In vivo biosensors: mechanisms, development, and applications.Journal of industrial microbiology & biotechnology · 2018Article
- Engineering 'cell robots' for parallel and highly sensitive screening of biomolecules under in vivo conditions.Scientific reports · 2017Article
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8 authors.
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Abstract
This study provided a new method which applied a selected L-lysine-inducible promoter for evolving lysine industrial strains of E. coli. According to the intracellular levels of the enhanced green fluorescent protein (EGFP) whose expression was controlled by the promoter, 186 strains were preliminarily selected using fluorescence-activated cell sorting from a 10-million-mutant library generated from a L-lysine high-producing E. coli strain. By subsequent multiple parameter evaluation of the 186 selected strains according to the concentration and the yield of lysine, the productivity per unit of cell in 96-deep-well blocks, two mutants MU-1 and MU-2 were obtained. They produced 136.51 ± 1.55 and 133.2 9 ± 1.42 g/L of lysine, respectively, in 5-L jars. Compared with the lysine concentration and the yield of the original strain, those of strain MU-1 improved by 21.00 and 9.05 %, respectively, and those of strain MU-2 improved by 18.14 and 10.41 %, respectively. The mutant selection and evaluation system newly established in our study should be useful for continuous improvement of the current E. coli strains in the lysine industry.
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