ArticleNucleic acids research2016
Combination probes with intercalating anchors and proximal fluorophores for DNA and RNA detection.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Modular and automated synthesis of oligonucleotide-small molecule conjugates for cathepsin B mediated traceless release of payloads.RSC chemical biology · 2024Article
- New Fluorophore and Its Applications in Visualizing Polystyrene Nanoplastics in Bean Sprouts and HeLa Cells.Molecules (Basel, Switzerland) · 2023Article
- Thermal stabilisation of the short DNA duplexes by acridine-4-carboxamide derivatives.Nucleic acids research · 2022Article
- Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.Bioconjugate chemistry · 2022Article
- A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA.Biochemistry · 2022Article
- Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions.Molecules (Basel, Switzerland) · 2021Review
- 5-Aza-7-deazaguanine-Isoguanine and Guanine-Isoguanine Base Pairs in Watson-Crick DNA: The Impact of Purine Tracts, Clickable Dendritic Side Chains, and Pyrene Adducts.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting.Organic & biomolecular chemistry · 2019Article
- Fluorophore-Quencher Interactions Effect on Hybridization Characteristics of Complementary Oligonucleotides.Analytical methods : advancing methods and applications · 2019Article
- An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA.Nature communications · 2019Article
- Fluorogenic PNA probes.Beilstein journal of organic chemistry · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
A new class of modified oligonucleotides (combination probes) has been designed and synthesised for use in genetic analysis and RNA detection. Their chemical structure combines an intercalating anchor with a reporter fluorophore on the same thymine nucleobase. The intercalator (thiazole orange or benzothiazole orange) provides an anchor, which upon hybridisation of the probe to its target becomes fluorescent and simultaneously stabilizes the duplex. The anchor is able to communicate via FRET to a proximal reporter dye (e.g. ROX, HEX, ATTO647N, FAM) whose fluorescence signal can be monitored on a range of analytical devices. Direct excitation of the reporter dye provides an alternative signalling mechanism. In both signalling modes, fluorescence in the unhybridised probe is switched off by collisional quenching between adjacent intercalator and reporter dyes. Single nucleotide polymorphisms in DNA and RNA targets are identified by differences in the duplex melting temperature, and the use of short hybridization probes, made possible by the stabilisation provided by the intercalator, enhances mismatch discrimination. Unlike other fluorogenic probe systems, placing the fluorophore and quencher on the same nucleobase facilitates the design of short probes containing multiple modifications. The ability to detect both DNA and RNA sequences suggests applications in cellular imaging and diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.