ArticleJournal of biomedical optics2016
Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope.
Article in Journal of biomedical optics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Review
- A general one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging.Nature communications · 2026Article
- OpenSTED: open-source dynamic intensity minimum system for stimulated emission depletion microscopy.Neurophotonics · 2024Article
- Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (International ed. in English) · 2023Article
- Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- Photobleaching reduction in modulated super-resolution microscopy.Microscopy (Oxford, England) · 2021Article
- Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy.International journal of molecular sciences · 2019Article
- Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.Cell reports · 2019Article
- AKAP150 Palmitoylation Regulates Synaptic Incorporation of CaCell reports · 2018Article
- Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines.eLife · 2017Article
- Determining the Spatial Relationship of Membrane-Bound Aquaporin-4 Autoantibodies by STED Nanoscopy.Biophysical journal · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We performed stimulated emission depletion (STED) imaging of isolated olfactory sensory neurons (OSNs) using a custom-built microscope. The STED microscope uses a single pulsed laser to excite two separate fluorophores, Atto 590 and Atto 647N. A gated timing circuit combined with temporal interleaving of the different color excitation/STED laser pulses filters the two channel detection and greatly minimizes crosstalk. We quantified the instrument resolution to be ∼81 and ∼44 nm, for the Atto 590 and Atto 647N channels. The spatial separation between the two channels was measured to be under 10 nm, well below the resolution limit. The custom-STED microscope is incorporated onto a commercial research microscope allowing brightfield, differential interference contrast, and epifluorescence imaging on the same field of view. We performed immunolabeling of OSNs in mice to image localization of ciliary membrane proteins involved in olfactory transduction. We imaged Ca2+-permeable cyclic nucleotide gated (CNG) channel (Atto 594) and adenylyl cyclase type III (ACIII) (Atto 647N) in distinct cilia. STED imaging resolved well-separated subdiffraction limited clusters for each protein. We quantified the size of each cluster to have a mean value of 88±48 nm and 124±43 nm, for CNG and ACIII, respectively. STED imaging showed separated clusters that were not resolvable in confocal images.
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Registered trials
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