Evidence map›Paper›PMID 27364703›Full record

ArticleScientific reports2016

Image Quality Ranking Method for Microscopy.

Sami Koho, Elnaz Fazeli, John E Eriksson, Pekka E Hänninen

Erratum issuedAbstract read
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

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  7. Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel-referenced Evaluation.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023
    Article
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  14. Multi-focus Image Fusion for Confocal Microscopy Using U-Net Regression Map.Proceedings of the ... IAPR International Conference on Pattern Recognition. International Conference on Pattern Recognition · 2021
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Sami KohoLaboratory of Biophysics, Department of Cell Biology and Anatomy, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Turku, Finland.
Elnaz FazeliLaboratory of Biophysics, Department of Cell Biology and Anatomy, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Turku, Finland.
John E ErikssonCell Biology, Biosciences, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland.
Pekka E HänninenLaboratory of Biophysics, Department of Cell Biology and Anatomy, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Turku, Finland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Automated analysis of microscope images is necessitated by the increased need for high-resolution follow up of events in time. Manually finding the right images to be analyzed, or eliminated from data analysis are common day-to-day problems in microscopy research today, and the constantly growing size of image datasets does not help the matter. We propose a simple method and a software tool for sorting images within a dataset, according to their relative quality. We demonstrate the applicability of our method in finding good quality images in a STED microscope sample preparation optimization image dataset. The results are validated by comparisons to subjective opinion scores, as well as five state-of-the-art blind image quality assessment methods. We also show how our method can be applied to eliminate useless out-of-focus images in a High-Content-Screening experiment. We further evaluate the ability of our image quality ranking method to detect out-of-focus images, by extensive simulations, and by comparing its performance against previously published, well-established microscopy autofocus metrics.

Identifiers

PMID27364703
PMCPMC4929473

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.