Evidence map›Paper›PMID 27364623›Full record

ArticleInfectious diseases of poverty2016

Assessing stool quantities generated by three specific Kato-Katz thick smear templates employed in different settings.

Andrea Leuenberger, Tatu Nassoro, Khadija Said, Lukas Fenner, George Sikalengo, Emilio Letang, Antonio Montresor, Xiao-Nong Zhou, Peter Steinmann, Hanspeter Marti and 2 more

Abstract readComparative Study
In one paragraph

Article in Infectious diseases of poverty, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Andrea LeuenbergerSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
Tatu NassoroBagamoyo Research and Training Centre, Bagamoyo District Hospital, Ifakara Health Institute, P.O. Box 74, Bagamoyo, United Republic of Tanzania.
Khadija SaidSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
Lukas FennerSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
George SikalengoIfakara Health Institute, Off Mlabani Passage, P.O. Box 53, Ifakara, United Republic of Tanzania.
Emilio LetangSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
Antonio MontresorDepartment of Control of Neglected Tropical Diseases, World Health Organization, Avenue Appia 20, CH-1211, Geneva 27, Switzerland.
Xiao-Nong ZhouChinese Center for Disease Control and Prevention, National Institute of Parasitic Diseases, 207 Rui Jin Er Road, Shanghai, 200025, People's Republic of China.
Peter SteinmannSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
Hanspeter MartiSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
Jürg UtzingerSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland.
Stefanie KnoppSwiss Tropical and Public Health Institute, P.O. Box, CH-4002, Basel, Switzerland. s.knopp@unibas.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe Kato-Katz technique is recommended for the diagnosis of helminth infections in epidemiological surveys, drug efficacy studies and monitoring of control interventions. We assessed the comparability of the average amount of faeces generated by three Kato-Katz templates included in test kits from two different providers.

methodsNine hundred Kato-Katz thick smear preparations were done; 300 per kit. Empty slides, slides plus Kato-Katz template filled with stool and slides plus stool after careful removal of the template were weighed to the nearest 0.1 mg. The average amount of stool that was generated on the slide was calculated for each template, stratified by standard categories of stool consistency (i.e. mushy, soft, sausage-shaped, hard and clumpy).

resultsThe average amount of stool generated on slides was 40.7 mg (95 % confidence interval (CI): 40.0-41.4 mg), 40.3 mg (95 % CI: 39.7-40.9 mg) and 42.8 mg (95 % CI: 42.2-43.3 mg) for the standard Vestergaard Frandsen template, and two different templates from the Chinese Center for Disease Control and Prevention (China CDC), respectively. Mushy stool resulted in considerably lower average weights when the Vestergaard Frandsen (37.0 mg; 95 % CI: 34.9-39.0 mg) or new China CDC templates (37.4 mg; 95 % CI: 35.9-38.9 mg) were used, compared to the old China CDC template (42.2 mg; 95 % CI: 40.7-43.7 mg) and compared to other stool consistency categories.

conclusionThe average amount of stool generated by three specific Kato-Katz templates was similar (40.3-42.8 mg). Since the multiplication factor is somewhat arbitrary and small changes only have little effect on infection intensity categories, it is suggested that the standard multiplication factor of 24 should be kept for the calculation of eggs per gram of faeces for all investigated templates.

Indexed as

AdolescentAdultAgedFecesHelminthiasisHumansLaboratoriesMiddle AgedParasite Egg CountParasitologySwitzerlandTanzaniaYoung AdultAccuracyDiagnosisInfection intensityKato-Katz techniqueSchistosomesSoil-transmitted helminths

Identifiers

PMID27364623
PMCPMC4929780

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.