ArticleACS chemical biology2016
Portability and Structure of the Four-Helix Bundle Docking Domains of trans-Acyltransferase Modular Polyketide Synthases.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Direct Quantification of Protein-Protein Interactions in Living Bacterial Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- An Integrated Module Performs Selective 'Online' Epoxidation in the Biosynthesis of the Antibiotic Mupirocin.Angewandte Chemie (International ed. in English) · 2024Article
- Insights into docking in megasynthases from the investigation of the toblerolRSC chemical biology · 2024Article
- The structure of a polyketide synthase bimodule core.Science advances · 2022Article
- Engineered biosynthesis of alkyne-tagged polyketides.Methods in enzymology · 2022Article
- Cooperation between a T Domain and a Minimal C-Terminal Docking Domain to Enable Specific Assembly in a Multiprotein NRPS.Angewandte Chemie (International ed. in English) · 2021Article
- Docking domain-mediated subunit interactions in natural product megasynth(et)ases.Journal of industrial microbiology & biotechnology · 2021Review
- Article
- Employing 25-Residue Docking Motifs from Modular Polyketide Synthases as Orthogonal Protein Connectors.ACS synthetic biology · 2019Article
- Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.ACS chemical biology · 2018Article
- The architectures of iterative type I PKS and FAS.Natural product reports · 2018Review
- Acyltransferases as Tools for Polyketide Synthase Engineering.Antibiotics (Basel, Switzerland) · 2018Review
- The modules of trans-acyltransferase assembly lines redefined with a central acyl carrier protein.Proteins · 2018Article
- Mechanism of intersubunit ketosynthase-dehydratase interaction in polyketide synthases.Nature chemical biology · 2018Article
- Structural and Functional Trends in Dehydrating Bimodules from trans-Acyltransferase Polyketide Synthases.Structure (London, England : 1993) · 2017Article
- The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.Chemical reviews · 2017Review
- Domain Organization and Active Site Architecture of a Polyketide Synthase C-methyltransferase.ACS chemical biology · 2016Article
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6 authors.
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Abstract
The polypeptides of multimodular polyketide synthases self-assemble into biosynthetic factories. While the docking domains that mediate the assembly of cis-acyltransferase polyketide synthase polypeptides are well-studied, those of the more recently discovered trans-acyltransferase polyketide synthases have just started to be described. Located at the C- and N-termini of many polypeptides, these 25-residue, two-helix, pseudosymmetric motifs noncovalently connect domains both between and within modules. Domains expressed with their natural, cognate docking motifs formed complexes stable to size-exclusion chromatography with 1-10 μM dissociation constants as measured by isothermal titration calorimetry. Deletion and swapping experiments demonstrate portability of the docking motifs. A 1.72 Å-resolution structure of the N-terminal portion of the macrolactin synthase polypeptide MlnE shows an uncomplexed N-terminal docking motif to be preorganized in the conformation it assumes within the docking domain complex.
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