ArticlePloS one2016
Assessment of Membrane Fluidity Fluctuations during Cellular Development Reveals Time and Cell Type Specificity.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
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- Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking.Cell reports · 2022Article
- In Silico Drug Design of Benzothiadiazine Derivatives Interacting with Phospholipid Cell Membranes.Membranes · 2022Article
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- Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.Frontiers in cell and developmental biology · 2021Article
- The Relationship between Erythrocytes and Diabetes Mellitus.Journal of diabetes research · 2021Review
- Bioglass could increase cell membrane fluidity with ion products to develop its bioactivity.Cell proliferation · 2020Article
- Lipidome alterations in human prefrontal cortex during development, aging, and cognitive disorders.Molecular psychiatry · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Cell membrane is made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as differentiation cell membranes undergo dramatic fluidity changes induced by proteins such as ARC and Cofilin among others. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. Membrane fluidity was measured at 12h, 72h and 92 h. Our results show significant changes in membrane fluidity among all cell types at different time points. GP values tend to increase significantly within 92 h in hN2 cells and 72 h in NIH3T3 cells and only at 92 h in HEK293 cells. L6 showed a marked decrease in membrane fluidity at 72 h and starts to increase at 92 h. As expected, NIH3T3 cells have more rigid membrane at earlier time points. On the other hand, neurons tend to have the highest membrane fluidity at early time points emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.
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