ArticlePloS one2016
Establishment of Nephrin Reporter Mice and Use for Chemical Screening.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 20 citations in OpenAlex.
- Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT.BMC genomics · 2024Article
- Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system.Experimental animals · 2020Article
- Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats.Neuron · 2019Article
- Generating mouse models for biomedical research: technological advances.Disease models & mechanisms · 2019Review
- Understanding Podocyte Biology to Develop Novel Kidney Therapeutics.Frontiers in endocrinology · 2018Review
- Pronuclear Injection-Based Targeted Transgenesis.Current protocols in human genetics · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nephrin is a critical component of glomerular filtration barrier, which is important to maintain glomerular structure and avoid proteinuria. Downregulation of nephrin expression is commonly observed at early stage of glomerular disorders, suggesting that methods to increase nephrin expression in podocytes may have therapeutic utility. Here, we generated a knockin mouse line carrying single copy of 5.5 kb nephrin promoter controlling expression of enhanced green fluorescent protein (EGFP) at Rosa26 genomic locus (Nephrin-EGFP mouse). In these mice, EGFP was specifically expressed in podocytes. Next, we isolated and cultivated glomeruli from these mice, and developed a protocol to automatically quantitate EGFP expression in cultured glomeruli. EGFP signal was markedly reduced after 5 days of culture but reduction was inhibited by vitamin D treatment. We confirmed that vitamin D increased mRNA and protein expression of endogenous nephrin in cultivated glomeruli. Thus, we generated a mouse line converting nephrin promoter activity into fluorescence, which can be used to screen compounds having activity to enhance nephrin gene expression.
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Registered trials
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