ArticlePloS one2016
Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
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- Time- and distance-resolved robotic imaging of fluid flow in vertical microfluidic strips: a new technique for quantitative, multiparameter measurement of global haemostasis.Sensors & diagnostics · 2023Article
- Automated, High-Throughput Analysis of Tire-DerivedACS ES&T water · 2023Article
- The Development of a 3D Printer-Inspired, Microgravity-Compatible Sample Preparation Device for Future Use Inside the International Space Station.Micromachines · 2023Article
- Performance Evaluation of a Novel Ultrafast Molecular Diagnostic Device Integrated With Microfluidic Chips and Dual Temperature Modules.Frontiers in bioengineering and biotechnology · 2022Article
- Parasitological, serological and molecular diagnosis of acute and chronic Chagas disease: from field to laboratory.Memorias do Instituto Oswaldo Cruz · 2022Article
- Miau, a microbalance autosampler.HardwareX · 2021Article
- Comparison of Design Approaches for Low-Cost Sampling Mechanisms in Open-Source Chemical Instrumentation.HardwareX · 2021Article
- How 3D printing can boost advances in analytical and bioanalytical chemistry.Mikrochimica acta · 2021Review
- Open-hardware wireless controller and 3D-printed pumps for efficient liquid manipulation.HardwareX · 2021Article
- Low-cost and open-source strategies for chemical separations.Journal of chromatography. A · 2021Review
- Integrated, Automated, Fast PCR System for Point-Of-Care Molecular Diagnosis of Bacterial Infection.Sensors (Basel, Switzerland) · 2021Article
- PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.PloS one · 2021Article
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource Settings.Diagnostics (Basel, Switzerland) · 2020Article
- Validating an Automated Nucleic Acid Extraction Device forFrontiers in microbiology · 2020Article
- Diagnostics in space: will zero gravity add weight to new advances?Expert review of molecular diagnostics · 2020Article
- Magnetic Particles for Advanced Molecular Diagnosis.Materials (Basel, Switzerland) · 2019Review
- Moving toward rapid and low-cost point-of-care molecular diagnostics with a repurposed 3D printer and RPA.Analytical biochemistry · 2018Article
- Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Most molecular diagnostic assays require upfront sample preparation steps to isolate the target's nucleic acids, followed by its amplification and detection using various nucleic acid amplification techniques. Because molecular diagnostic methods are generally rather difficult to perform manually without highly trained users, automated and integrated systems are highly desirable but too costly for use at point-of-care or low-resource settings. Here, we showcase the development of a low-cost and rapid nucleic acid isolation and amplification platform by modifying entry-level 3D printers that cost between $400 and $750. Our modifications consisted of replacing the extruder with a tip-comb attachment that houses magnets to conduct magnetic particle-based nucleic acid extraction. We then programmed the 3D printer to conduct motions that can perform high-quality extraction protocols. Up to 12 samples can be processed simultaneously in under 13 minutes and the efficiency of nucleic acid isolation matches well against gold-standard spin-column-based extraction technology. Additionally, we used the 3D printer's heated bed to supply heat to perform water bath-based polymerase chain reactions (PCRs). Using another attachment to hold PCR tubes, the 3D printer was programmed to automate the process of shuttling PCR tubes between water baths. By eliminating the temperature ramping needed in most commercial thermal cyclers, the run time of a 35-cycle PCR protocol was shortened by 33%. This article demonstrates that for applications in resource-limited settings, expensive nucleic acid extraction devices and thermal cyclers that are used in many central laboratories can be potentially replaced by a device modified from inexpensive entry-level 3D printers.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.