Evidence map›Paper›PMID 27358894›Full record

ArticleStem cell investigation2016

Adipose-derived stem cells from lean and obese humans show depot specific differences in their stem cell markers, exosome contents and senescence: role of protein kinase C delta (PKCδ) in adipose stem cell niche.

Rekha S Patel, Gay Carter, Ghattas El Bassit, Achintya A Patel, Denise R Cooper, Michel Murr, Niketa A Patel

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In one paragraph

Article in Stem cell investigation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 57 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Rekha S Patel1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
Gay Carter1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
Ghattas El Bassit1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
Achintya A Patel1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
Denise R Cooper1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
Michel Murr1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
Niketa A Patel1 Department of Molecular Medicine, University of South Florida, Tampa, FL, USA ; 2 James A. Haley Veterans Hospital, Tampa, FL, USA ; 3 Honors College, University of South Florida, Tampa, FL, USA ; 4 Department of Surgery, University of South Florida, Tampa, FL, USA.
University of South Florida · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAdipose-derived stem cells (ASC) and its exosomes are gaining utmost importance in the field of regenerative medicine. The ASCs tested for their potential in wound healing are predominantly derived from the subcutaneous depot of lean donors. However, it is important to characterize the ASC derived from different adipose depots as these depots have clinically distinct roles.

methodsWe characterized the ASC derived from subcutaneous and omental depots from a lean donor (sc-ASCn and om-ASCn) and compared it to the ASC derived from an obese donor (sc-ASCo and om-ASCo) using flow cytometry and real time qPCR.

resultsWe show that stem cell markers Oct4, Sal4, Sox15, KLF4 and BMI1 have distinct expression patterns in each ASC. We evaluated the secretome of the ASC and characterized their secreted exosomes. We show long noncoding RNAs (lncRNAs) are secreted by ASC and their expression varied between the ASC's derived from different depots. Protein kinase C delta (PKCδ) regulates the mitogenic signals in stem cells. We evaluated the effect of silencing PKCδ in sc-ASCn, om-ASCn, sc-ASCo and om-ASCo. Using β-galactosidase staining, we evaluated the percentage of senescent cells in sc-ASCn, om-ASCn, sc-ASCo and om-ASCo. Our results also indicated that silencing PKCδ increases the percentage of senescent cells.

conclusionsOur case-specific study demonstrates a role of PKCδ in maintaining the adipose stem cell niche and importantly demonstrates depot-specific differences in adipose stem cells and their exosome content.

Indexed as

Adipose-derived stem cells (ASCs)adipose stem cell markersexosomelong noncoding RNAs (lncRNA)obesityProtein kinase C delta (PKCδ)senescence

Identifiers

PMID27358894
PMCPMC4923648
OpenAlexW2260661980

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.