ArticleJournal of the Royal Society, Interface2016
Self-organization of chemoattractant waves in Dictyostelium depends on F-actin and cell-substrate adhesion.
Article in Journal of the Royal Society, Interface, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- From microscope to model: rotating signaling dynamics and cluster size in Dictyostelium discoideum aggregates.BMC molecular and cell biology · 2026Article
- Traveling-wave chemotaxis of neutrophil-like HL-60 cells.Molecular biology of the cell · 2025Article
- Imaging-Based Analysis of Cell-Cell Contact-Dependent Migration in Dictyostelium.Methods in molecular biology (Clifton, N.J.) · 2024Article
- An integrated, cross-regulation pathway model involving activating/adaptive and feed-forward/feed-back loops for directed oscillatory cAMP signal-relay/response during the development ofFrontiers in cell and developmental biology · 2023Article
- Spatiotemporal development of coexisting wave domains of Rho activity in the cell cortex.Scientific reports · 2021Article
- Rap1 Is Essential for B-Cell Locomotion, Germinal Center Formation and Normal B-1a Cell Population.Frontiers in immunology · 2021Article
- UsingFrontiers in cell and developmental biology · 2021Review
- Phosphatidic acid-dependent localization and basal de-phosphorylation of RA-GEFs regulate lymphocyte trafficking.BMC biology · 2020Article
- Article
- Interplay of mesoscale physics and agent-like behaviors in the parallel evolution of aggregative multicellularity.EvoDevo · 2020Review
- GPCR-controlled membrane recruitment of negative regulator C2GAP1 locally inhibits Ras signaling for adaptation and long-range chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Fold-change detection and scale invariance of cell-cell signaling in social amoeba.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
In the social amoeba Dictyostelium discoideum, travelling waves of extracellular cyclic adenosine monophosphate (cAMP) self-organize in cell populations and direct aggregation of individual cells to form multicellular fruiting bodies. In contrast to the large body of studies that addressed how movement of cells is determined by spatial and temporal cues encoded in the dynamic cAMP gradients, how cell mechanics affect the formation of a self-generated chemoattractant field has received less attention. Here, we show, by live cell imaging analysis, that the periodicity of the synchronized cAMP waves increases in cells treated with the actin inhibitor latrunculin. Detail analysis of the extracellular cAMP-induced transients of cytosolic cAMP (cAMP relay response) in well-isolated cells demonstrated that their amplitude and duration were markedly reduced in latrunculin-treated cells. Similarly, in cells strongly adhered to a poly-l-lysine-coated surface, the response was suppressed, and the periodicity of the population-level oscillations was markedly lengthened. Our results suggest that cortical F-actin is dispensable for the basic low amplitude relay response but essential for its full amplification and that this enhanced response is necessary to establish high-frequency signalling centres. The observed F-actin dependence may prevent aggregation centres from establishing in microenvironments that are incompatible with cell migration.
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