ArticleAngewandte Chemie (International ed. in English)2016
Digital Quantification of DNA Replication and Chromosome Segregation Enables Determination of Antimicrobial Susceptibility after only 15 Minutes of Antibiotic Exposure.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 94 citations in OpenAlex.
- A lab-on-a-silicon-chip platform for all-electrical antibiotic susceptibility tests with a sample-to-results time within 20 minutes.Science advances · 2026Article
- Rapid and quantitative measurement of bacteriophage infectivity via fully automated droplet digital PCR.Nature communications · 2026Article
- Near-Infrared-Responsive Digital PCR-Assisted Renal Cancer Exosomal miRNAs Insights and Regulation of Macrophage Polarization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Exploiting β-Lactams-Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual-Digital PCR.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Next-generation rapid phenotypic antimicrobial susceptibility testing.Nature communications · 2024Review
- Phenotypic and genotypic perspectives on detection methods for bacterial antimicrobial resistance in a One Health context: research progress and prospects.Archives of microbiology · 2024Review
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay.Antibiotics (Basel, Switzerland) · 2023Article
- Trends of respiratory virus detection in point-of-care testing: A review.Analytica chimica acta · 2023Review
- Activated alkyne-enabled turn-on click bioconjugation with cascade signal amplification for ultrafast and high-throughput antibiotic screening.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2022Review
- Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium.Journal of visualized experiments : JoVE · 2022Article
- Microfluidic Systems for Antimicrobial Susceptibility Testing.Advances in biochemical engineering/biotechnology · 2022Article
- Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition.Scientific reports · 2021Article
- Multiplexed droplet loop-mediated isothermal amplification with scorpion-shaped probes and fluorescence microscopic counting for digital quantification of virus RNAs.Chemical science · 2021Article
- Recent Development of Rapid Antimicrobial Susceptibility Testing Methods through Metabolic Profiling of Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Affordable automated phenotypic antibiotic susceptibility testing method based on a contactless conductometric sensor.Scientific reports · 2020Article
- ddRFC: A scalable multiplexed droplet digital nucleic acid amplification test platform.Biosensors & bioelectronics · 2020Article
- Rapid Determination of Antimicrobial Susceptibility by Stimulated Raman Scattering Imaging of DAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level.Chemical science · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Rapid antimicrobial susceptibility testing (AST) would decrease misuse and overuse of antibiotics. The "holy grail" of AST is a phenotype-based test that can be performed within a doctor visit. Such a test requires the ability to determine a pathogen's susceptibility after only a short antibiotic exposure. Herein, digital PCR (dPCR) was employed to test whether measuring DNA replication of the target pathogen through digital single-molecule counting would shorten the required time of antibiotic exposure. Partitioning bacterial chromosomal DNA into many small volumes during dPCR enabled AST results after short exposure times by 1) precise quantification and 2) a measurement of how antibiotics affect the states of macromolecular assembly of bacterial chromosomes. This digital AST (dAST) determined susceptibility of clinical isolates from urinary tract infections (UTIs) after 15 min of exposure for all four antibiotic classes relevant to UTIs. This work lays the foundation to develop a rapid, point-of-care AST and strengthen global antibiotic stewardship.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.