ArticleScientific reports2016
Microfluidics-based side view flow chamber reveals tether-to-sling transition in rolling neutrophils.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Article
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- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- A humanized βCell reports · 2022Article
- Mitofusin-2 regulates leukocyte adhesion and β2 integrin activation.Journal of leukocyte biology · 2022Article
- Bone Marrow Transplantation Rescues Monocyte Recruitment Defect and Improves Cystic Fibrosis in Mice.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Single-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling.Communications biology · 2021Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis.The Journal of experimental medicine · 2021Article
- Opportunities and Challenges in Tunneling Nanotubes Research: How Far from Clinical Application?International journal of molecular sciences · 2021Review
- Modeling membrane nanotube morphology: the role of heterogeneity in composition and material properties.Scientific reports · 2020Article
- Modular microfluidic systems cast from 3D-printed molds for imaging leukocyte adherence to differentially treated endothelial cultures.Scientific reports · 2019Article
- Neutrophil Recruitment: From Model Systems to Tissue-Specific Patterns.Trends in immunology · 2019Review
- A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry.Journal of visualized experiments : JoVE · 2019Article
- High-Affinity Bent βCell reports · 2019Article
- Rolling neutrophils form tethers and slings under physiologic conditions in vivo.Journal of leukocyte biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Neutrophils rolling at high shear stress (above 6 dyn/cm(2)) form tethers in the rear and slings in the front. Here, we developed a novel photo-lithographically fabricated, silicone(PDMS)-based side-view flow chamber to dynamically visualize tether and sling formation. Fluorescently membrane-labeled mouse neutrophils rolled on P-selectin substrate at 10 dyn/cm(2). Most rolling cells formed 5 tethers that were 2-30 μm long. Breaking of a single tether caused a reproducible forward microjump of the cell, showing that the tether was load-bearing. About 15% of all tether-breaking events resulted in slings. The tether-to-sling transition was fast (<100 ms) with no visible material extending above the rolling cell, suggesting a very low bending modulus of the tether. The sling downstream of the rolling cell aligned according to the streamlines before landing on the flow chamber. These new observations explain how slings form from tethers and provide insight into their biomechanical properties.
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Registered trials
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