ArticleProceedings of the National Academy of Sciences of the United States of America2016
Mapping transcription factor interactome networks using HaloTag protein arrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
- Extranuclear function of Arabidopsis HOOKLESS1 regulates pleiotropic developmental processes in a non-cell-autonomous manner.The New phytologist · 2025Article
- In Vitro GT-array (Bio-protocol · 2024Article
- Harmony of Protein Tags and Chimeric Molecules Empowers Targeted Protein Ubiquitination and Beyond.Cells · 2024Review
- Mapping adipocyte interactome networks by HaloTag-enrichment-mass spectrometry.Biology methods & protocols · 2024Article
- Review
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- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- New insights into the role of chrysanthemum calcineurin B-like interacting protein kinase CmCIPK23 in nitrate signaling in Arabidopsis roots.Scientific reports · 2022Article
- A plant-specific syntaxin-6 protein contributes to the intracytoplasmic route for the begomovirus CabLCV.Plant physiology · 2021Article
- A multilayered cross-species analysis of GRAS transcription factors uncovered their functional networks in plant adaptation to the environment.Journal of advanced research · 2021Article
- ConnecTF: A platform to integrate transcription factor-gene interactions and validate regulatory networks.Plant physiology · 2021Article
- Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root Gravitropism.Current biology : CB · 2021Article
- New Insights on the Regulation of Glucosinolate BiosynthesisFrontiers in plant science · 2021Article
- Improving coordination of plant growth and nitrogen metabolism for sustainable agriculture.aBIOTECH · 2020Review
- Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag.Bio-protocol · 2020Article
- Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP-Tag Technologies.Chembiochem : a European journal of chemical biology · 2020Review
- Nitrate in 2020: Thirty Years from Transport to Signaling Networks.The Plant cell · 2020Review
- Regulatory Potential of bHLH-Type Transcription Factors on the Road to Rubber Biosynthesis inPlants (Basel, Switzerland) · 2020Article
- Time-Course Transcriptome Study Reveals Mode of bZIP Transcription Factors on Light Exposure inInternational journal of molecular sciences · 2020Article
- HaloTag-based conjugation of proteins to barcoding-oligonucleotides.Nucleic acids research · 2020Article
Corrections and comments
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Authors and funding
23 authors at 7 institutions in 4 countries.
Funding
Abstract
Protein microarrays enable investigation of diverse biochemical properties for thousands of proteins in a single experiment, an unparalleled capacity. Using a high-density system called HaloTag nucleic acid programmable protein array (HaloTag-NAPPA), we created high-density protein arrays comprising 12,000 Arabidopsis ORFs. We used these arrays to query protein-protein interactions for a set of 38 transcription factors and transcriptional regulators (TFs) that function in diverse plant hormone regulatory pathways. The resulting transcription factor interactome network, TF-NAPPA, contains thousands of novel interactions. Validation in a benchmarked in vitro pull-down assay revealed that a random subset of TF-NAPPA validated at the same rate of 64% as a positive reference set of literature-curated interactions. Moreover, using a bimolecular fluorescence complementation (BiFC) assay, we confirmed in planta several interactions of biological interest and determined the interaction localizations for seven pairs. The application of HaloTag-NAPPA technology to plant hormone signaling pathways allowed the identification of many novel transcription factor-protein interactions and led to the development of a proteome-wide plant hormone TF interactome network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.