ArticleScientific reports2016
Cold acclimation wholly reorganizes the Drosophila melanogaster transcriptome and metabolome.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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86 citing papers in PubMed.
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- Heat stress drives opposing redox shifts in temperate versus tropicalbioRxiv : the preprint server for biology · 2026Article
- Transcriptomic responses of red palm weevil (Coleoptera: Curculionidae) larvae to low-temperature exposure.Journal of insect science (Online) · 2026Article
- Sex-Specific Regulation of the Turandot Gene Family Modulates Temperature-Dependent Lifespan in Drosophila melanogaster.Aging cell · 2026Article
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- Cold adaptation mechanism in Bactrocera dorsalis (Diptera: Tephritidae) by regulating MAPK signaling and metabolic pathways.Journal of insect science (Online) · 2026Article
- Lipid Properties and Metabolism in Response to Cold.Advances in experimental medicine and biology · 2026Review
- Invasive African fig flies (microPublication biology · 2026Article
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- Single-nucleus multiome ATAC and RNA sequencing reveals the molecular basis of thermal acclimation inbioRxiv : the preprint server for biology · 2025Article
- Divergence and Parallelism in Two Tropical Drosophilids Simultaneously Invading a Desert Environment.Genome biology and evolution · 2025Article
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- Navigating the Cold: Integrative Transcriptome Sequencing Approach Reveals Ionoregulatory and Whole-Body Responses to Cold Acclimation in Drosophila ananassae.Genome biology and evolution · 2025Article
- Revealing the Immune Response ofInsects · 2024Article
- Microbial-transcriptome integrative analysis of heat stress effects on amino acid metabolism and lipid peroxidation in poultry jejunum.Animal biotechnology · 2024Article
- Metabolite changes by combined treatment, ethyl formate and low temperature, in Drosophila suzukii.Scientific reports · 2024Article
- Decoding functional proteome information in model organisms using protein language models.NAR genomics and bioinformatics · 2024Article
- Biochemical indicators, cell apoptosis, and metabolomic analyses of the low-temperature stress response and cold tolerance mechanisms in Litopenaeus vannamei.Scientific reports · 2024Article
26 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
Cold tolerance is a key determinant of insect distribution and abundance, and thermal acclimation can strongly influence organismal stress tolerance phenotypes, particularly in small ectotherms like Drosophila. However, there is limited understanding of the molecular and biochemical mechanisms that confer such impressive plasticity. Here, we use high-throughput mRNA sequencing (RNA-seq) and liquid chromatography - mass spectrometry (LC-MS) to compare the transcriptomes and metabolomes of D. melanogaster acclimated as adults to warm (rearing) (21.5 °C) or cold conditions (6 °C). Cold acclimation improved cold tolerance and led to extensive biological reorganization: almost one third of the transcriptome and nearly half of the metabolome were differentially regulated. There was overlap in the metabolic pathways identified via transcriptomics and metabolomics, with proline and glutathione metabolism being the most strongly-supported metabolic pathways associated with increased cold tolerance. We discuss several new targets in the study of insect cold tolerance (e.g. dopamine signaling and Na(+)-driven transport), but many previously identified candidate genes and pathways (e.g. heat shock proteins, Ca(2+) signaling, and ROS detoxification) were also identified in the present study, and our results are thus consistent with and extend the current understanding of the mechanisms of insect chilling tolerance.
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