ArticleActa veterinaria Scandinavica2016
Optimized methods for extracting circulating small RNAs from long-term stored equine samples.
Article in Acta veterinaria Scandinavica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids.Veterinary research communications · 2023Article
- Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys.Journal of veterinary internal medicine · 2021Article
- Diagnostic and prognostic potential of eight whole blood microRNAs for equine sarcoid disease.PloS one · 2021Article
- Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression.Journal of veterinary internal medicine · 2019Article
- Identification of Reference Genes for Analysis of microRNA Expression Patterns in Equine Chorioallantoic Membrane and Serum.Molecular biotechnology · 2018Article
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- Circulating microRNAs as Potential Biomarkers of Infectious Disease.Frontiers in immunology · 2017Review
- Novel equine tissue miRNAs and breed-related miRNA expressed in serum.BMC genomics · 2016Article
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Authors and funding
5 authors.
Funding
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Abstract
Circulating miRNAs in body fluids, particularly serum, are promising candidates for future routine biomarker profiling in various pathologic conditions in human and veterinary medicine. However, reliable standardized methods for miRNA extraction from equine serum and fresh or archived whole blood are sorely lacking. We systematically compared various miRNA extraction methods from serum and whole blood after short and long-term storage without addition of RNA stabilizing additives prior to freezing. Time of storage at room temperature prior to freezing did not affect miRNA quality in serum. Furthermore, we showed that miRNA of NGS-sufficient quality can be recovered from blood samples after >10 years of storage at -80 °C. This allows retrospective analyses of miRNAs from archived samples.
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