ArticleAngewandte Chemie (International ed. in English)2016
Supramolecular Control over Split-Luciferase Complementation.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 66 citations in OpenAlex.
- Supramolecularly gated enzymes: On-demand activity switching via host-guest competition for precision tumor therapy.Materials today. Bio · 2026Article
- Modular protein frameworks via supramolecular synthons.Biophysical reviews · 2025Review
- Peptide recognition by a synthetic receptor at subnanomolar concentrations.Chemical science · 2024Article
- Cucurbit[8]uril Binds Nonterminal Dipeptide Sites with High Affinity and Induces a Type II β-Turn.Journal of the American Chemical Society · 2024Article
- Molecular Approaches to Protein Dimerization: Opportunities for Supramolecular Chemistry.Frontiers in chemistry · 2022Review
- Design and recognition of cucurbituril-secured platinum-bound oligopeptides.Chemical science · 2021Article
- Toward Light-Controlled Supramolecular Peptide Dimerization.The Journal of organic chemistry · 2021Article
- Light-driven release of cucurbit[8]uril from a bivalent cage.Chemical science · 2021Article
- Facile Fabrication of Protein-Macrocycle Frameworks.Journal of the American Chemical Society · 2021Article
- Self Assembled Cages with Mechanically Interlocked Cucurbiturils.Supramolecular chemistry · 2021Article
- Modular bioengineered kinase sensorsChemical science · 2020Article
- Discovery of Influenza Polymerase PA-PB1 Interaction Inhibitors Using anACS chemical biology · 2020Article
- Article
- Functional protein nanostructures: a chemical toolbox.Chemical Society reviews · 2018Review
- Cucurbit[8]uril Reactivation of an Inactivated Caspase-8 Mutant Reveals Differentiated Enzymatic Substrate Processing.Chembiochem : a European journal of chemical biology · 2018Article
- Supramolecular Chemistry Targeting Proteins.Journal of the American Chemical Society · 2017Article
- Host-Guest Tethered DNA Transducer: ATP Fueled Release of a Protein Inhibitor from Cucurbit[7]uril.Journal of the American Chemical Society · 2017Article
- A Binary Bivalent Supramolecular Assembly Platform Based on Cucurbit[8]uril and Dimeric Adapter Protein 14-3-3.Angewandte Chemie (International ed. in English) · 2017Article
- Synthetic mimics of biotin/(strept)avidin.Chemical Society reviews · 2017Review
- Assessment of Cooperativity in Ternary Peptide-Cucurbit[8]uril Complexes.Chemistry (Weinheim an der Bergstrasse, Germany) · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Supramolecular split-enzyme complementation restores enzymatic activity and allows for on-off switching. Split-luciferase fragment pairs were provided with an N-terminal FGG sequence and screened for complementation through host-guest binding to cucurbit[8]uril (Q8). Split-luciferase heterocomplex formation was induced in a Q8 concentration dependent manner, resulting in a 20-fold upregulation of luciferase activity. Supramolecular split-luciferase complementation was fully reversible, as revealed by using two types of Q8 inhibitors. Competition studies with the weak-binding FGG peptide revealed a 300-fold enhanced stability for the formation of the ternary heterocomplex compared to binding of two of the same fragments to Q8. Stochiometric binding by the potent inhibitor memantine could be used for repeated cycling of luciferase activation and deactivation in conjunction with Q8, providing a versatile module for in vitro supramolecular signaling networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.