ArticlePloS one2016
A Conserved MicroRNA Regulatory Circuit Is Differentially Controlled during Limb/Appendage Regeneration.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Material-Assisted Strategies for Osteochondral Defect Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Pooled it
- MiRNA Atlas: A Literature-Derived Database of MicroRNAs Bridging Osteoarthritis and Appendage Regeneration.bioRxiv : the preprint server for biology · 2026Article
- MicroRNAs in Tissue Regeneration: Lessons from Animal Models.International journal of molecular sciences · 2025Review
- Anabolic indices of matrix proteins identify regenerative small RNA intrinsic to human cartilage.Science advances · 2025Article
- The mRNA and microRNA Landscape of the Blastema Niche in Regenerating Newt Limbs.International journal of molecular sciences · 2024Article
- Arthritis Foundation/HSS Workshop on Hip Osteoarthritis, Part 2: Detecting Hips at Risk: Early Biomechanical and Structural Mechanisms.HSS journal : the musculoskeletal journal of Hospital for Special Surgery · 2023Review
- Expression Analysis ofInternational journal of molecular sciences · 2022Article
- Biological properties of the BCL-2 family protein BCL-RAMBO, which regulates apoptosis, mitochondrial fragmentation, and mitophagy.Frontiers in cell and developmental biology · 2022Review
- Self-Control of Inflammation during Tail Regeneration of Lizards.Journal of developmental biology · 2021Review
- Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super-Regenerator Vertebrates to Combat Scarring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Genetic, Epigenetic, and Post-Transcriptional Basis of Divergent Tissue Regenerative Capacities Among Vertebrates.Advanced genetics (Hoboken, N.J.) · 2021Article
- BCL2L13: physiological and pathological meanings.Cellular and molecular life sciences : CMLS · 2021Review
- Divergent Influenza-Like Viruses of Amphibians and Fish Support an Ancient Evolutionary Association.Viruses · 2020Article
- Emerging Concepts and Challenges in Rheumatoid Arthritis Gene Therapy.Biomedicines · 2020Review
- Functional Characterization of the Lin28/let-7 Circuit During Forelimb Regeneration inFrontiers in cell and developmental biology · 2020Article
- Integrative Analysis of Axolotl Gene Expression Data from Regenerative and Wound Healing Limb Tissues.Scientific reports · 2019Article
- A Tale of Two States: Normal and Transformed, With and Without Rigidity Sensing.Annual review of cell and developmental biology · 2019Review
- Analysis of "old" proteins unmasks dynamic gradient of cartilage turnover in human limbs.Science advances · 2019Article
- High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Profiling microRNA expression in Atlantic killifish (Fundulus heteroclitus) gill and responses to arsenic and hyperosmotic stress.Aquatic toxicology (Amsterdam, Netherlands) · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough regenerative capacity is evident throughout the animal kingdom, it is not equally distributed throughout evolution. For instance, complex limb/appendage regeneration is muted in mammals but enhanced in amphibians and teleosts. The defining characteristic of limb/appendage regenerative systems is the formation of a dedifferentiated tissue, termed blastema, which serves as the progenitor reservoir for regenerating tissues. In order to identify a genetic signature that accompanies blastema formation, we employ next-generation sequencing to identify shared, differentially regulated mRNAs and noncoding RNAs in three different, highly regenerative animal systems: zebrafish caudal fins, bichir pectoral fins and axolotl forelimbs.
resultsThese studies identified a core group of 5 microRNAs (miRNAs) that were commonly upregulated and 5 miRNAs that were commonly downregulated, as well as 4 novel tRNAs fragments with sequences conserved with humans. To understand the potential function of these miRNAs, we built a network of 1,550 commonly differentially expressed mRNAs that had functional relationships to 11 orthologous blastema-associated genes. As miR-21 was the most highly upregulated and most highly expressed miRNA in all three models, we validated the expression of known target genes, including the tumor suppressor, pdcd4, and TGFβ receptor subunit, tgfbr2 and novel putative target genes such as the anti-apoptotic factor, bcl2l13, Choline kinase alpha, chka and the regulator of G-protein signaling, rgs5.
conclusionsOur extensive analysis of RNA-seq transcriptome profiling studies in three regenerative animal models, that diverged in evolution ~420 million years ago, reveals a common miRNA-regulated genetic network of blastema genes. These comparative studies extend our current understanding of limb/appendage regeneration by identifying previously unassociated blastema genes and the extensive regulation by miRNAs, which could serve as a foundation for future functional studies to examine the process of natural cellular reprogramming in an injury context.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.