ArticlePLoS neglected tropical diseases2016
Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00803621 (Cerebral Toxoplasmosis and AIDS in the French Departments of America), which is not on this map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Cerebral Toxoplasmosis and AIDS in the French Departments of America (DFA). Diagnostic Contribution of a PCR Assay and Genetic Diversity of Toxoplasma Gondii.
Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Toxoplasmosis Acquired in French Guiana in Immunocompetent Adults: Case Series From 2002 to 2019, With a Focus on Outpatients.Open forum infectious diseases · 2026Article
- Cerebral toxoplasmosis in the twenty-first century.AIDS (London, England) · 2026Observational
- Exposure of Wild Ruminants toVeterinary sciences · 2025Article
- Toxoplasma gondii from Gabonese forest, Central Africa: First report of an African wild strain.PLoS neglected tropical diseases · 2025Article
- Molecular diagnosis of human toxoplasmosis: the state of the art.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Review
- In Vitro Evaluation of Anti-Parasitic Activities of Quinolone-Coumarin Hybrids Derived from Fluoroquinolones and Novobiocin Against Toxoplasma gondii.Acta parasitologica · 2024Article
- Exposure of Urban European Hedgehogs (Animals : an open access journal from MDPI · 2024Article
- Licochalcone A: a review of its pharmacology activities and molecular mechanisms.Frontiers in pharmacology · 2024Review
- First detection ofFrontiers in cellular and infection microbiology · 2024Article
- Validation of the NAT Chagas IVD Kit for the Detection and Quantification ofLife (Basel, Switzerland) · 2023Article
- Lack of Detection ofPathogens (Basel, Switzerland) · 2022Article
- A Field-Deployable Insulated Isothermal PCR (iiPCR) for the Global Surveillance ofAnimals : an open access journal from MDPI · 2022Article
- Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin.Parasite (Paris, France) · 2022Article
- Toxoplasma gondii in the faeces of wild felids from the Atlantic Forest, Brazil.Memorias do Instituto Oswaldo Cruz · 2022Article
- Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay.PLoS neglected tropical diseases · 2021Article
- Serological Responses to Toxoplasma gondii and Matrix Antigen 1 Predict the Risk of Subsequent Toxoplasmic Encephalitis in People Living With Human Immunodeficiency Virus (HIV).Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Detection of toxoplasmic encephalitis in HIV positive patients in urine with hydrogel nanoparticles.PLoS neglected tropical diseases · 2021Article
- PCR-based diagnosis is not always useful in the acute acquired toxoplasmosis in immunocompetent individuals.Parasitology research · 2021Article
- Human toxoplasmosis in Mozambique: gaps in knowledge and research opportunities.Parasites & vectors · 2020Review
- Approach to an Intracranial Mass in Patients With HIV.Current neurology and neuroscience reports · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 9 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundToxoplasmic encephalitis in patients with AIDS is a life-threatening disease mostly due to reactivation of Toxoplasma gondii cysts in the brain. The main objective of this study was to evaluate the performance of real-time PCR assay in peripheral blood samples for the diagnosis of toxoplasmic encephalitis in AIDS patients in the French West Indies and Guiana. METHODOLOGY/PRINCIPAL
findingsAdult patients with HIV and suspicion of toxoplasmic encephalitis with start of specific antitoxoplasmic therapy were included in this study during 40 months. The real-time PCR assay targeting the 529 bp repeat region of T. gondii was performed in two different centers for all blood samples. A Neighbor-Joining tree was reconstructed from microsatellite data to examine the relationships between strains from human cases of toxoplasmosis in South America and the Caribbean. A total of 44 cases were validated by a committee of experts, including 36 cases with toxoplasmic encephalitis. The specificity of the PCR assay in blood samples was 100% but the sensitivity was only 25% with moderate agreement between the two centers. Altered level of consciousness and being born in the French West Indies and Guiana were the only two variables that were associated with significantly decreased risk of false negative results with the PCR assay. CONCLUSION/SIGNIFICANCE: Our results showed that PCR sensitivity in blood samples increased with severity of toxoplasmic encephalitis in AIDS patients. Geographic origin of patients was likely to influence PCR sensitivity but there was little evidence that it was caused by differences in T. gondii strains.
trial registrationClinicalTrials.gov NCT00803621.
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Registered trials
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