ArticlePloS one2016
Generation of Anti-Boa Immunoglobulin Antibodies for Serodiagnostic Applications, and Their Use to Detect Anti-Reptarenavirus Antibodies in Boa Constrictor.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Boid inclusion body disease iniScience · 2026Article
- Snake kolmiovirus encodes a single form of delta antigen and shows no evidence of translation from ORF2.The Journal of general virology · 2026Article
- Temperature affects reptarenavirus growth in a permissive host-derivedThe Journal of general virology · 2025Article
- Persistent Reptarenavirus and Hartmanivirus Infection in Cultured Boid Cells.Microbiology spectrum · 2022Article
- Article
- Experimental Reptarenavirus Infection ofJournal of virology · 2021Article
- Nidoviruses in Reptiles: A Review.Frontiers in veterinary science · 2021Review
- Orthohantavirus Isolated in Reservoir Host Cells Displays Minimal Genetic Changes and Retains Wild-Type Infection Properties.Viruses · 2020Article
- Differences in Tissue and Species Tropism of Reptarenavirus Species Studied by Vesicular Stomatitis Virus Pseudotypes.Viruses · 2020Article
- Snake Deltavirus Utilizes Envelope Proteins of Different Viruses To Generate Infectious Particles.mBio · 2020Article
- Serological and molecular findings during SARS-CoV-2 infection: the first case study in Finland, January to February 2020.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
- Prevalence of inclusion body disease and associated comorbidity in captive collections of boid and pythonid snakes in Belgium.PloS one · 2020Article
- Article
- Characterization of Haartman Institute snake virus-1 (HISV-1) and HISV-like viruses-The representatives of genus Hartmanivirus, family Arenaviridae.PLoS pathogens · 2018Article
- Nidovirus-Associated Proliferative Pneumonia in the Green Tree Python (Morelia viridis).Journal of virology · 2017Article
- Co-infecting Reptarenaviruses Can Be Vertically Transmitted in Boa Constrictor.PLoS pathogens · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunoglobulins (Igs), the key effectors of the adaptive immune system, mediate the specific recognition of foreign structures, i.e. antigens. In mammals, IgM production commonly precedes the production of IgG in the response to an infection. The reptilian counterpart of IgG is IgY, but the exact kinetics of the reptilian immune response are less well known. Boid inclusion body disease (BIBD), an often fatal disease of captive boas and pythons has been linked to reptarenavirus infection, and BIBD is believed to be immunosuppressive. However, so far, the study of the serological response towards reptarenaviruses in BIBD has been hampered by the lack of reagents. Thus we set up a purification protocol for boa constrictor IgY and IgM, which should also be applicable for other snake species. We used centrifugal filter units, poly ethylene glycol precipitation and gel permeation chromatography to purify and separate the IgM and IgY fractions from boa constrictor serum, which we further used to immunise rabbits. We affinity purified IgM and IgY specific reagents from the produced antiserum, and labelled the reagents with horseradish peroxidase. Finally, using the sera of snakes with known exposure to reptarenaviruses we demonstrated that the newly generated reagents can be utilised for serodiagnostic purposes, such as immunoblotting and immunofluorescent staining. To our knowledge, this is the first report to show reptarenavirus-specific antibodies in boa constrictors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.