ArticlePloS one2016
Targeted Mutagenesis of the Hypophysiotropic Gnrh3 in Zebrafish (Danio rerio) Reveals No Effects on Reproductive Performance.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 99 citations in OpenAlex.
- The Role of Neurohypophysial Hormones in the Endocrine and Paracrine Control of Gametogenesis in Fish.Cells · 2025Review
- Sterilization of Channel Catfish (Ictalurus punctatus) via Overexpression of bax Gene Regulated by a Tet-off System in the Primordial Germ Cells.Marine biotechnology (New York, N.Y.) · 2025Article
- The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion.eLife · 2024Article
- GnRH-Gonadotropes Interactions Revealed by Pituitary Single-cell Transcriptomics in Zebrafish.Endocrinology · 2024Article
- Somatostatin signalling coordinates energy metabolism allocation to reproduction in zebrafish.BMC biology · 2024Article
- Loss of Function of Vasoactive-intestinal Peptide Alters Sex Ratio and Reduces Male Reproductive Fitness in Zebrafish.Endocrinology · 2024Article
- Identification of the FSH-RH as the other gonadotropin-releasing hormone.Nature communications · 2024Article
- Reversible Sterilization of Channel Catfish via Overexpression of Glutamic Acid Decarboxylase Gene.Animals : an open access journal from MDPI · 2024Article
- Chemogenetic Depletion of Hypophysiotropic GnRH Neurons Does Not Affect Fertility in Mature Female Zebrafish.International journal of molecular sciences · 2022Article
- Article
- Tachykinins, new players in the control of reproduction and food intake: A comparative review in mammals and teleosts.Frontiers in endocrinology · 2022Review
- Identification and expression analysis of zebrafishFrontiers in genetics · 2022Article
- Overview and New Insights Into the Diversity, Evolution, Role, and Regulation of Kisspeptins and Their Receptors in Teleost Fish.Frontiers in endocrinology · 2022Review
- The application of genome editing technology in fish.Marine life science & technology · 2021Review
- Gnrh2 maintains reproduction in fasting zebrafish through dynamic neuronal projection changes and regulation of gonadotropin synthesis, oogenesis, and reproductive behaviors.Scientific reports · 2021Article
- Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction.Science advances · 2021Article
- Reduced Vitellogenesis and Female Fertility in Gper Knockout Zebrafish.Frontiers in endocrinology · 2021Article
- Fish reproductive biology - Reflecting on five decades of fundamental and translational research.General and comparative endocrinology · 2021Review
- Targeted mutation of secretogranin-2 disrupts sexual behavior and reproduction in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42.Open biology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gnrh is the major neuropeptide regulator of vertebrate reproduction, triggering a cascade of events in the pituitary-gonadal axis that result in reproductive competence. Previous research in mice and humans has demonstrated that Gnrh/GNRH null mutations result in hypogonadotropic hypogonadism and infertility. The goal of this study was to eliminate gnrh3 (the hypophysiotropic Gnrh form) function in zebrafish (Danio rerio) to determine how ontogeny and reproductive performance are affected, as well as factors downstream of Gnrh3 along the reproductive axis. Using the TALEN technology, we developed a gnrh3-/- zebrafish line that harbors a 62 bp deletion in the gnrh3 gene. Our gnrh3-/- zebrafish line represents the first targeted and heritable mutation of a Gnrh isoform in any organism. Using immunohistochemistry, we verified that gnrh3-/- fish do not possess Gnrh3 peptide in any regions of the brain. However, other than changes in mRNA levels of pituitary gonadotropin genes (fshb, lhb, and cga) during early development, which are corrected by adulthood, there were no changes in ontogeny and reproduction in gnrh3-/- fish. The gnrh3-/- zebrafish are fertile, displaying normal gametogenesis and reproductive performance in males and females. Together with our previous results that Gnrh3 cell ablation causes infertility, these results indicate that a compensatory mechanism is being activated, which is probably primed early on upon Gnrh3 neuron differentiation and possibly confined to Gnrh3 neurons. Potential compensation factors and sensitive windows of time for compensation during development and puberty should be explored.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.