ArticleThe Plant cell2016
A Sorghum Mutant Resource as an Efficient Platform for Gene Discovery in Grasses.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 125 citations in OpenAlex.
- A grass-specific structural myosin VIII regulates protoxylem development and hydraulic conductance in sorghum.Plant physiology · 2026Article
- The SbTDR gene is required for anther development and male fertility in Sorghum bicolor.Plant reproduction · 2026Article
- SorghumBase: a knowledgebase for sorghum genomics, phenomics, and stakeholder engagement.Genetics · 2026Article
- Gramene 2025: expanded comparative genomics and pathway resources, integrated search, and pan-genome portals for crop research.Nucleic acids research · 2026Article
- Effects of a novel loss-of-function waxy endosperm allele on sorghum seed development and grain quality.G3 (Bethesda, Md.) · 2025Article
- Rice grain size: current regulatory mechanisms and future perspectives.Journal of plant research · 2025Review
- Progress and innovations of gene cloning in wheat and its close relatives.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Sorghum as a monocot model for drought research.Frontiers in plant science · 2025Review
- Untargeted mutagenesis of brassinosteroid receptor SbBRI1 confers drought tolerance by altering phenylpropanoid metabolism in Sorghum bicolor.Plant biotechnology journal · 2024Article
- Article
- Genomic variation induced by a low concentration of ethyl methanesulfonate (EMS) in quinoa 'Longli-4' variety.Botanical studies · 2024Article
- Mapping and Screening of Candidate Gene Regulating the Biomass Yield of Sorghum (International journal of molecular sciences · 2024Article
- A large-scale whole-exome sequencing mutant resource for functional genomics in wheat.Plant biotechnology journal · 2023Article
- Mutagenesis in the Age of Next-Generation-Sequencing and Genome Editing.Plants (Basel, Switzerland) · 2023Article
- Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment.aBIOTECH · 2023Article
- Variations in Total Protein and Amino Acids in the Sequenced Sorghum Mutant Library.Plants (Basel, Switzerland) · 2023Article
- Variation in mitogenome structural conformation in wild and cultivated lineages of sorghum corresponds with domestication history and plastome evolution.BMC plant biology · 2023Article
- Article
- Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.Frontiers in genetics · 2023Review
- Whole-Genome Sequencing of Rice Mutant Library Members Induced by N-Methyl-N-Nitrosourea Mutagenesis of Fertilized Egg Cells.Rice (New York, N.Y.) · 2022Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorghum (Sorghum bicolor) is a versatile C4 crop and a model for research in family Poaceae. High-quality genome sequence is available for the elite inbred line BTx623, but functional validation of genes remains challenging due to the limited genomic and germplasm resources available for comprehensive analysis of induced mutations. In this study, we generated 6400 pedigreed M4 mutant pools from EMS-mutagenized BTx623 seeds through single-seed descent. Whole-genome sequencing of 256 phenotyped mutant lines revealed >1.8 million canonical EMS-induced mutations, affecting >95% of genes in the sorghum genome. The vast majority (97.5%) of the induced mutations were distinct from natural variations. To demonstrate the utility of the sequenced sorghum mutant resource, we performed reverse genetics to identify eight genes potentially affecting drought tolerance, three of which had allelic mutations and two of which exhibited exact cosegregation with the phenotype of interest. Our results establish that a large-scale resource of sequenced pedigreed mutants provides an efficient platform for functional validation of genes in sorghum, thereby accelerating sorghum breeding. Moreover, findings made in sorghum could be readily translated to other members of the Poaceae via integrated genomics approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.