ArticleScientific reports2016
Subsurface Super-resolution Imaging of Unstained Polymer Nanostructures.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Advances in the mechanism of small extracellular vesicles promoting the development of hepatocellular carcinoma through multi-network fusion.Frontiers in immunology · 2025Review
- Single Molecules Are Your Quanta: A Bottom-Up Approach toward Multidimensional Super-resolution Microscopy.ACS nano · 2021Review
- Super-Resolution Imaging of Self-Assembled Nanocarriers Using Quantitative Spectroscopic Analysis for Cluster Extraction.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Multicolor super-resolution imaging using spectroscopic single-molecule localization microscopy with optimal spectral dispersion.Applied optics · 2019Article
- Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy.Journal of biomedical optics · 2018Article
- Spectroscopic analysis beyond the diffraction limit.The international journal of biochemistry & cell biology · 2018Review
- Spatial and spectral imaging of point-spread functions using a spatial light modulator.Optics communications · 2017Article
- Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Super-resolution spectroscopic microscopy via photon localization.Nature communications · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Optical imaging has offered unique advantages in material researches, such as spectroscopy and lifetime measurements of deeply embedded materials, which cannot be matched using electron or scanning-probe microscopy. Unfortunately, conventional optical imaging cannot provide the spatial resolutions necessary for many nanoscopic studies. Despite recent rapid progress, super-resolution optical imaging has yet to be widely applied to non-biological materials. Herein we describe a method for nanoscopic optical imaging of buried polymer nanostructures without the need for extrinsic staining. We observed intrinsic stochastic fluorescence emission or blinking from unstained polymers and performed spatial-temporal spectral analysis to investigate its origin. We further applied photon localization super-resolution imaging reconstruction to the detected stochastic blinking, and achieved a spatial resolution of at least 100 nm, which corresponds to a six-fold increase over the optical diffraction limit. This work demonstrates the potential for studying the static heterogeneities of intrinsic polymer molecular-specific properties at sub-diffraction-limited optical resolutions.
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Registered trials
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