Evidence map›Paper›PMID 27353647›Full record

ArticleScientific reports2016

The infectious BAC genomic DNA expression library: a high capacity vector system for functional genomics.

Michele M P Lufino, Pauline A H Edser, Michael A Quail, Stephen Rice, David J Adams, Richard Wade-Martins

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Michele M P LufinoDepartment of Physiology, Anatomy and Genetics, University of Oxford, Le Gros Clark Building, South Parks Road, Oxford, OX1 3QX, UK.
Pauline A H EdserDepartment of Physiology, Anatomy and Genetics, University of Oxford, Le Gros Clark Building, South Parks Road, Oxford, OX1 3QX, UK.
Michael A QuailWellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK.
Stephen RiceWellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK.
David J AdamsWellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK.
Richard Wade-MartinsDepartment of Physiology, Anatomy and Genetics, University of Oxford, Le Gros Clark Building, South Parks Road, Oxford, OX1 3QX, UK.

Funding

Biotechnology and Biological Sciences Research Council BB/D012759/1Biotechnology and Biological Sciences Research Council BB/D012910/1Cancer Research UKWellcome Trust
6 · The paper itself

Abstract

Gene dosage plays a critical role in a range of cellular phenotypes, yet most cellular expression systems use heterologous cDNA-based vectors which express proteins well above physiological levels. In contrast, genomic DNA expression vectors generate physiologically-relevant levels of gene expression by carrying the whole genomic DNA locus of a gene including its regulatory elements. Here we describe the first genomic DNA expression library generated using the high-capacity herpes simplex virus-1 amplicon technology to deliver bacterial artificial chromosomes (BACs) into cells by viral transduction. The infectious BAC (iBAC) library contains 184,320 clones with an average insert size of 134.5 kb. We show in a Chinese hamster ovary (CHO) disease model cell line and mouse embryonic stem (ES) cells that this library can be used for genetic rescue studies in a range of contexts including the physiological restoration of Ldlr deficiency, and viral receptor expression. The iBAC library represents an important new genetic analysis tool openly available to the research community.

Indexed as

Gene ExpressionGene LibraryAnimalsCHO CellsChromosomes, Artificial, BacterialCricetulusEscherichia coliHerpesvirus 1, HumanMiceMouse Embryonic Stem CellsTransduction, Genetic

Identifiers

PMID27353647
PMCPMC4926088

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.