ArticleNature communications2016
Droplet barcoding for massively parallel single-molecule deep sequencing.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Review
- A droplet microfluidics-based platform for generating target-specific, natively-paired immune libraries and identifying potent and developable antibodies.Scientific reports · 2026Article
- Light-Responsive Materials in Droplet Manipulation for Biochemical Applications.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Automating life science labs at the single-cell level through precise ultrasonic liquid sample ejection: PULSE.Microsystems & nanoengineering · 2024Article
- Targeted whole-genome recovery of single viral species in a complex environmental sample.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeted phasing of 2-200 kilobase DNA fragments with a short-read sequencer and a single-tube linked-read library method.Scientific reports · 2024Article
- Massively parallel single-cell sequencing of diverse microbial populations.Nature methods · 2024Article
- Deep mutational scanning of proteins in mammalian cells.Cell reports methods · 2023Review
- Review
- A Review of Optical Imaging Technologies for Microfluidics.Micromachines · 2022Review
- Pico-washing: simultaneous liquid addition and removal for continuous-flow washing of microdroplets.Microsystems & nanoengineering · 2022Article
- Microdrop Human Immunodeficiency Virus Sequencing for Incidence and Drug Resistance Surveillance.The Journal of infectious diseases · 2021Article
- Integration of Droplet Microfluidic Tools for Single-Cell Functional Metagenomics: An Engineering Head Start.Genomics, proteomics & bioinformatics · 2021Article
- Screening of Additive Formulations Enables Off-Chip Drop Reverse Transcription Quantitative Polymerase Chain Reaction of Single Influenza A Virus Genomes.Analytical chemistry · 2021Article
- Generation of Nanodroplet Reactors and Their Applications in In Situ Controllable Synthesis and Transportation of Ag Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Deterministic droplet codingLab on a chip · 2020Article
- Targeted Single-Cell RNA and DNA Sequencing With Fluorescence-Activated Droplet Merger.Analytical chemistry · 2020Article
- Unravelling the complex genetics of common kidney diseases: from variants to mechanisms.Nature reviews. Nephrology · 2020Review
- Facile Production of Large-Area Cell Arrays Using Surface-Assembled Microdroplets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Limited DNA Sequencing Based on Tube and Droplet.Micromachines · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The ability to accurately sequence long DNA molecules is important across biology, but existing sequencers are limited in read length and accuracy. Here, we demonstrate a method to leverage short-read sequencing to obtain long and accurate reads. Using droplet microfluidics, we isolate, amplify, fragment and barcode single DNA molecules in aqueous picolitre droplets, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read sequencing. We show that this approach can provide accurate sequences of up to 10 kb, allowing us to identify rare mutations below the detection limit of conventional sequencing and directly link them into haplotypes. This barcoding methodology can be a powerful tool in sequencing heterogeneous populations such as viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.