ArticleNucleic acids research2016
Homology directed repair is unaffected by the absence of siRNAs in Drosophila melanogaster.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- RNA polymerase II is recruited to DNA double-strand breaks for dilncRNA transcription inRNA biology · 2022Article
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- Single-molecule imaging of transcription at damaged chromatin.Science advances · 2019Article
- The Role of Noncoding RNAs in Double-Strand Break Repair.Frontiers in plant science · 2019Review
- Beyond the Trinity of ATM, ATR, and DNA-PK: Multiple Kinases Shape the DNA Damage Response in Concert With RNA Metabolism.Frontiers in molecular biosciences · 2019Review
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair.Nature communications · 2018Article
- Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.Nature cell biology · 2017Article
- Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.Nucleic acids research · 2017Article
- Splicing stimulates siRNA formation at Drosophila DNA double-strand breaks.PLoS genetics · 2017Article
- Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The emerging role of RNAs in DNA damage repair.Cell death and differentiation · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small interfering RNAs (siRNAs) defend the organism against harmful transcripts from exogenous (e.g. viral) or endogenous (e.g. transposons) sources. Recent publications describe the production of siRNAs induced by DNA double-strand breaks (DSB) in Neurospora crassa, Arabidopsis thaliana, Drosophila melanogaster and human cells, which suggests a conserved function. A current hypothesis is that break-induced small RNAs ensure efficient homologous recombination (HR). However, biogenesis of siRNAs is often intertwined with other small RNA species, such as microRNAs (miRNAs), which complicates interpretation of experimental results. In Drosophila, siRNAs are produced by Dcr-2 while miRNAs are processed by Dcr-1. Thus, it is possible to probe siRNA function without miRNA deregulation. We therefore examined DNA double-strand break repair after perturbation of siRNA biogenesis in cultured Drosophila cells as well as mutant flies. Our assays comprised reporters for the single-strand annealing pathway, homologous recombination and sensitivity to the DSB-inducing drug camptothecin. We could not detect any repair defects caused by the lack of siRNAs derived from the broken DNA locus. Since production of these siRNAs depends on local transcription, they may thus participate in RNA metabolism-an established function of siRNAs-rather than DNA repair.
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