ArticleVeterinary research2016
"Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella.
Article in Veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Abnormal expression pattern of knock-in marker in Eimeria tenella using CRISPR/Cas9.Poultry science · 2026Article
- RNG2 tethers the conoid to the apical polar ring in Toxoplasma gondii to enable parasite motility and invasion.PLoS biology · 2025Article
- Development of SARS-CoV-2 as a viral vector: A novel intranasal bivalent vaccine for SARS-CoV-2 and RSV.Science advances · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.Veterinary research · 2024Article
- Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3.Veterinary research · 2024Article
- In vivo thrombin activity in the diatom Phaeodactylum tricornutum: biotechnological insights.Applied microbiology and biotechnology · 2024Article
- PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods.The EMBO journal · 2024Article
- Developing efficient strategies for localizing the enhanced yellow fluorescent protein subcellularly in transgenic Eimeria parasites.Scientific reports · 2024Article
- Microneme-located VP2 inInfection and immunity · 2024Article
- The VP1 Protein of Porcine Teschovirus Inhibits the Innate Immune Response to Viral Infection by Blocking MDA5 Activation.Transboundary and emerging diseases · 2024Article
- Expression of IL-1β in transgenicFrontiers in immunology · 2024Article
- Oral delivery ofFrontiers in veterinary science · 2024Article
- Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.Nature biomedical engineering · 2023Article
- Engineering Rapalog-Inducible Genetic Switches Based on Split-T7 Polymerase to Regulate Oncolytic Virus-Driven Production of Tumour-Localized IL-12 for Anti-Cancer Immunotherapy.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Article
- Enhancement of ergothioneine production by discovering and regulating its metabolic pathway in Cordyceps militaris.Microbial cell factories · 2022Article
- Delineating a serotonin 1B receptor circuit for appetite suppression in mice.The Journal of experimental medicine · 2022Article
- The Construction and Immunogenicity Analyses of Recombinant Pseudorabies Virus With NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus-Like Particles Co-expression.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The "self-cleaving" 2A sequence of picornavirus, which mediates ribosome-skipping events, enables the generation of two or more separate peptide products from one mRNA containing one or more "self-cleaving" 2A sequences. In this study, we introduced a single 2A sequence of porcine teschovirus-1 (P2A) linked to two fluorescent protein genes, the enhanced yellow fluorescent protein (EYFP) gene and the red fluorescent protein (RFP) gene, in a single cassette into transgenic Eimeria tenella (EtER). As expected, we obtained two separated protein molecules rather than a fused protein, although the two molecules were translated from the same mRNA carrying a single "self-cleaving" 2A sequence. Importantly, RFP led by a secretion signal was secreted into parasitophorous vacuoles, while EYFP localized mainly to the nucleus of EtER. Our results demonstrate that the "self-cleaving" 2A sequence actively mediated cleavage of polyproteins in the apicomplexan parasite E. tenella.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.