ArticleScientific reports2016
DNA barcoding, species-specific PCR and real-time PCR techniques for the identification of six Tribolium pests of stored products.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 37 citations in OpenAlex.
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- A genomic approach for accurate identification of closely related species with next-generation sequencing samples.Scientific reports · 2026Article
- The relationship between taxonomic classification and applied entomology: stored product pests as a model group.Journal of insect science (Online) · 2025Review
- Detection and molecular identification of scrub typhus vectors and pathogens from rice field rats, a traditional food item of Mizo tribes in Mizoram, Northeast India.BMC veterinary research · 2025Article
- Main Chemical Components, Activity and Mechanism of Repellence ofMolecules (Basel, Switzerland) · 2025Article
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- DNA barcoding for the identification and authentication of medicinal deer (Cervus sp.) products in China.PloS one · 2024Article
- Species-specific markers for3 Biotech · 2023Article
- Effect of high temperature on Wolbachia density and impact on cytoplasmic incompatibility in confused flour beetle, Tribolium confusum (Coleoptera: Tenebrionidae).BMC research notes · 2022Article
- Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species inMitochondrial DNA. Part B, Resources · 2019Article
- A protocol for obtaining DNA barcodes from plant and insect fragments isolated from forensic-type soils.International journal of legal medicine · 2018Article
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored product pests in China and worldwide. They are often found or are intercepted in grain depots, flour mills, and entry-exit ports, etc. Traditionally, Tribolium species are identified according to the morphological characteristics of the adult. However, it is almost impossible to rapidly identify adult fragments and non-adult stages based on external morphological characteristics. Molecular techniques for the rapid and accurate identification of Tribolium species are required, particularly for pest monitoring and the quarantine of stored products pests. Here, we establish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six stored-product pest Tribolium species including T. castaneum, T. confusum, T. destructor, T. madens, T. freemani and T. brevicornis. We detected the mitochondrial DNA cytochrome oxidase subunit I (COI) barcodes for Tribolium from 18 geographic populations and 101 individuals, built a Tribolium DNA barcode library, and designed species-specific primers and TaqMan probes for the above six Tribolium species. The three techniques were applied to identify Tribolium collected from stored samples and samples captured from quarantine ports. The results demonstrated that three techniques were all able to identify the six species of Tribolium both rapidly and accurately.
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