ArticleBMC research notes2016
Simultaneous purification of DNA and RNA from microbiota in a single colonic mucosal biopsy.
Article in BMC research notes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
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- Efficacy of oral and rectal administration of human faecal microbiota transplant (FMT) in human microbiota-associated mouse models.Scientific reports · 2025Article
- Microbial Patterns in Newly Diagnosed Inflammatory Bowel Disease Revealed by Presence and Transcriptional Activity - Relationship to Diagnosis and Outcome.Clinical and experimental gastroenterology · 2025Article
- Transcriptomic and proteomic profiling reveal immune and metabolic dysregulation in the colonic mucosa of people living with HIV with incomplete immune recovery.Frontiers in immunology · 2025Article
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- Establishment of a consensus protocol to explore the brain pathobiome in patients with mild cognitive impairment and Alzheimer's disease: Research outline and call for collaboration.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2023Review
- Sample Collection Methods in Upper Gastrointestinal Research.Journal of Korean medical science · 2023Review
- Hyperglycemia is associated with duodenal dysbiosis and altered duodenal microenvironment.Scientific reports · 2023Article
- Optimized bacterial DNA isolation method for microbiome analysis of human tissues.MicrobiologyOpen · 2021Article
- Lipid antigens in bile from patients with chronic liver diseases activate natural killer T cells.Clinical and experimental immunology · 2021Article
- Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer.Scientific reports · 2021Article
- Detection of Fusobacterium nucleatum in stool and colonic tissues from Norwegian colorectal cancer patients.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Fecal microbiota profiles in treatment-naïve pediatric inflammatory bowel disease - associations with disease phenotype, treatment, and outcome.Clinical and experimental gastroenterology · 2019Article
- The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients.Scientific reports · 2018Article
- Recent insights into the tick microbiome gained through next-generation sequencing.Parasites & vectors · 2018Review
- Characterization of the fecal and mucosa-associated microbiota in dogs with colorectal epithelial tumors.PloS one · 2018Article
- Exploring the human microbiome from multiple perspectives: factors altering its composition and function.FEMS microbiology reviews · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNucleic acid purification methods are of importance when performing microbiota studies and especially when analysing the intestinal microbiota as we here find a wide range of different microbes. Various considerations must be taken to lyse the microbial cell wall of each microbe. In the present article, we compare several tissue lysis steps and commercial purification kits, to achieve a joint RNA and DNA purification protocol for the purpose of investigating the microbiota and the microbiota-host interactions in a single colonic mucosal tissue sample.
resultsA further optimised tissue homogenisation and lysis protocol comprising mechanical bead beating, lysis buffer replacement and enzymatic treatment, in combination with the AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) resulted in efficient and simultaneous purification of microbial and human RNA and DNA from a single mucosal colonic tissue sample.
conclusionsThe present work provides a unique possibility to study RNA and DNA from the same mucosal biopsy sample, making a direct comparison between metabolically active microbes and total microbial DNA. The protocol also offers an opportunity to investigate other members of a microbiota such as viruses, fungi and micro-eukaryotes, and moreover the possibility to extract data on microbiota and host interactions from one single mucosal biopsy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.