ArticleJournal of biomolecular NMR2016
High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles.
Article in Journal of biomolecular NMR, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- High-frequency dynamic nuclear polarization in rotating solids.Science advances · 2026Review
- Thermal Evolution of Scallop and Surf Clam Shell: Phase Transformation Insights from Solid-StateMaterials (Basel, Switzerland) · 2026Article
- Isoleucine Side Chains as Reporters of Conformational Freedom in Protein Folding Studied by DNP-Enhanced NMR.Journal of the American Chemical Society · 2025Article
- Resolution in cryogenic solid state NMR: Challenges and solutions.Protein science : a publication of the Protein Society · 2024Review
- A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity.Chemical science · 2023Article
- Conformational ensembles explain NMR spectra of frozen intrinsically disordered proteins.Protein science : a publication of the Protein Society · 2023Article
- PyrroTriPol: a semi-rigid trityl-nitroxide for high field dynamic nuclear polarization.Chemical science · 2023Article
- Biradical Polarizing Agents at High Fields.The journal of physical chemistry. B · 2022Article
- DNP-enhanced solid-state NMR spectroscopy of chromatin polymers.Journal of magnetic resonance open · 2022Article
- Article
- Characterization of photonic band resonators for DNP NMR of thin film samples at 7 T magnetic field.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2021Article
- Competing Transfer Pathways in Direct and Indirect Dynamic Nuclear Polarization MAS NMR Experiments on HIV-1 Capsid Assemblies: Implications for Sensitivity and Resolution.Magnetic resonance (Gottingen, Germany) · 2021Article
- Targetable Tetrazine-Based Dynamic Nuclear Polarization Agents for Biological Systems.Chembiochem : a European journal of chemical biology · 2020Article
- Modular, triple-resonance, transmission line DNP MAS probe for 500 MHz/330 GHz.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2019Article
- High-sensitivity protein solid-state NMR spectroscopy.Current opinion in structural biology · 2019Review
- High frequency dynamic nuclear polarization: New directions for the 21st century.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2019Review
- Direct dynamic nuclear polarization ofSolid state nuclear magnetic resonance · 2019Article
- Dynamic Nuclear Polarization Magic-Angle Spinning Nuclear Magnetic Resonance Combined with Molecular Dynamics Simulations Permits Detection of Order and Disorder in Viral Assemblies.The journal of physical chemistry. B · 2019Article
- Article
- Multi-resonant photonic band-gap/saddle coil DNP probehead for static solid state NMR of microliter volume samples.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800 MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22 %, indicating that inhomogeneous broadening is not the dominant effect that limits the resolution of T3SS needles under DNP conditions. The outstanding spectral resolution of this system under DNP conditions can be attributed to its low overall flexibility.
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