ArticleGenes and environment : the official journal of the Japanese Environmental Mutagen Society2015
Genomic integration of lambda EG10 transgene in gpt delta transgenic rodents.
Article in Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Mutagenicity of carcinogenic heterocyclic amines in Salmonella typhimurium YG strains and transgenic rodents including gpt delta.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Review
- Characteristic mutations induced in the small intestine of Msh2-knockout gpt delta mice.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- New homozygous gpt delta transgenic rat strain improves an efficiency of the in vivo mutagenicity assay.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Modulation ofChemical research in toxicology · 2020Article
- DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote.Nucleic acids research · 2020Article
- Effects of theGenes and environment : the official journal of the Japanese Environmental Mutagen Society · 2020Article
- Mutagenicity of Helicobacter hepaticus infection in the lower bowel mucosa of 129/SvEv Rag2International journal of cancer · 2019Article
- Thresholds of Genotoxic and Non-Genotoxic Carcinogens.Toxicological research · 2018Review
- Change over time of the mutagenicity in the lungs ofGenes and environment : the official journal of the Japanese Environmental Mutagen Society · 2018Article
- Transgenic rat models for mutagenesis and carcinogenesis.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2017Review
- Estimation of the frequency of inherited germline mutations by whole exome sequencing in ethyl nitrosourea-treated and untreated gpt delta mice.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransgenic gpt delta mouse and rat models were developed to perform gpt and Spi(-) assays for in vivo mutagenicity tests. The animals were established by integration of lambda EG10 phage DNA as a transgene into the genome. The inserted position of the transgene on chromosome was determined by fluorescent in situ hybridization and Southern blot analyses; however, the exact position and sequence of the inserted junction were not known. To identify the site and pattern of genomic integration of the transgene copies, genomic DNAs extracted from C57BL/6J gpt delta mice and F344 gpt delta rats were applied to whole genome sequencing and mate-pair analysis.
resultsThe result confirmed that multi-copy lambda EG10 transgenes are inserted at a single position in the mouse chromosome 17. The junction contains 70 bp of overlapped genomic sequences, and it has short homology at both ends. A copy number analysis suggested that the inserted transgenes may contain 41 head-to-tail junctions and 16 junctions of other types such as rearranged abnormal junctions. It suggested that the number of intact copies could be approximately 40 at maximum. In the F344 gpt delta rats, transgenes are inserted at a single position in the rat chromosome 4. The junction contains no overlapped sequence but 72-kb genomic sequence including one gene was deleted. The inserted transgenes may contain 15 head-to-tail junctions and two rearranged junctions. It suggested that the number of intact copies could be 14 at maximum. One germline base substitution in the gpt gene rescued from gpt delta rats was characterized.
conclusionsThe exact inserted positions of the lambda EG10 transgene in the genome of gpt delta transgenic rodents were identified. The copy number and arrangement of the transgene were analyzed. PCR primers for quick genotyping of gpt delta mice and rats have been designed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.