ArticleMicrobial cell factories2016
Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 33 citations in OpenAlex.
- A new paradigm for the regulation of A40926B0 biosynthesis.Synthetic and systems biotechnology · 2025Article
- A VioA Variant Activates Antibiotic Streptogramins in the Heterologous HostMarine drugs · 2025Article
- Heterologous expression facilitates the discovery and characterization of marine microbial natural products.Engineering microbiology · 2024Review
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- Elicitation for activation of the actinomycete genome's cryptic secondary metabolite gene clusters.RSC advances · 2023Review
- Genetic Engineering ofMicroorganisms · 2021Article
- WblA, a global regulator of antibiotic biosynthesis in Streptomyces.Journal of industrial microbiology & biotechnology · 2021Review
- Eliciting the silent lucensomycin biosynthetic pathway in Streptomyces cyanogenus S136 via manipulation of the global regulatory gene adpA.Scientific reports · 2021Article
- Metagenomics Approaches in Discovery and Development of New Bioactive Compounds from Marine Actinomycetes.Current microbiology · 2020Review
- Secondary nucleotide messenger c-di-GMP exerts a global control on natural product biosynthesis in streptomycetes.Nucleic acids research · 2020Article
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Regulation of antibiotic biosynthesis in actinomycetes: Perspectives and challenges.Synthetic and systems biotechnology · 2018Review
- Activation of microbial secondary metabolic pathways: Avenues and challenges.Synthetic and systems biotechnology · 2018Review
- Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-DerivedMarine drugs · 2018Article
- Overexpression of a type III PKS gene affording novel violapyrones with enhanced anti-influenza A virus activity.Microbial cell factories · 2018Article
- Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters.Microbial cell factories · 2018Article
- Discovery of Antimycin-Type Depsipeptides from aFrontiers in microbiology · 2017Article
- A New Dioic Acid from a wbl Gene Mutant of Deepsea-Derived Streptomyces somaliensis SCSIO ZH66.Marine drugs · 2016Article
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundActinomycete genome sequencing has disclosed a large number of cryptic secondary metabolite biosynthetic gene clusters. However, their unavailable or limited expression severely hampered the discovery of bioactive compounds. The whiB-like (wbl) regulatory genes play important roles in morphological differentiation as well as secondary metabolism; and hence the wblA so gene was probed and set as the target to activate cryptic gene clusters in deepsea-derived Streptomyces somaliensis SCSIO ZH66.
resultswblA so from deepsea-derived S. somaliensis SCSIO ZH66 was inactivated, leading to significant changes of secondary metabolites production in the ΔwblA so mutant, from which α-pyrone compound violapyrone B (VLP B) was isolated. Subsequently, the VLP biosynthetic gene cluster was identified and characterized, which consists of a type III polyketide synthase (PKS) gene vioA and a regulatory gene vioB; delightedly, inactivation of vioB led to isolation of another four VLPs analogues, among which one was new and two exhibited improved anti-MRSA (methicillin-resistant Staphylococcus aureus, MRSA) activity than VLP B. Moreover, transcriptional analysis revealed that the expression levels of whi genes (whiD, whiG, whiH and whiI) and wbl genes (wblC, wblE, wblH, wblI and wblK) were repressed by different degrees, suggesting an intertwined regulation mechanism of wblA so in morphological differentiation and secondary metabolism of S. somaliensis SCSIO ZH66.
conclusionswblA orthologues would be effective targets for activation of cryptic gene clusters in marine-derived Streptomyces strains, notwithstanding the regulation mechanisms might be varied in different strains. Moreover, the availability of the vio gene cluster has enriched the diversity of type III PKSs, providing new opportunities to expand the chemical space of polyketides through biosynthetic engineering.
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