ArticleParasites & vectors2016
Functional analysis of Orco and odorant receptors in odor recognition in Aedes albopictus.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile.PloS one · 2026Article
- A plant virus manipulates both its host plant and the insect that facilitates its transmission.Science advances · 2025Article
- Phenotypic variation in biting behavior associated with differences in expression of olfactory genes in the vector mosquito, Aedes albopictus (Diptera: Culicidae).Journal of medical entomology · 2024Article
- High-throughput ligand profile characterization in novel cell lines expressing seven heterologous insect olfactory receptors for the detection of volatile plant biomarkers.Scientific reports · 2023Article
- Odor-evoked transcriptomics of Aedes aegypti mosquitoes.PloS one · 2023Article
- Article
- The chemosensory system of theFly · 2022Review
- Genome-wide identification and expression profiling of odorant receptor genes in the malaria vector Anopheles sinensis.Parasites & vectors · 2022Article
- Screening for odorant receptor genes expressed in Aedes aegypti involved in host-seeking, blood-feeding and oviposition behaviors.Parasites & vectors · 2022Article
- Molecular and functional characterization of a conserved odorant receptor from Aedes albopictus.Parasites & vectors · 2022Article
- Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.Scientific reports · 2021Article
- Design of a Repellent Against Aedes aegypti (Diptera: Culicidae) Using in silico Simulations With AaegOBP1 Protein.Journal of medical entomology · 2020Article
- An odorant receptor from Anopheles sinensis in China is sensitive to oviposition attractants.Malaria journal · 2018Article
- Odorant Receptors and Odorant-Binding Proteins as Insect Pest Control Targets: A Comparative Analysis.Frontiers in physiology · 2018Review
- Expressional and functional interactions of twoPeerJ · 2018Article
- Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus.BMC genomics · 2017Article
- Identification and expression analysis of chemosensory receptor genes in an aphid endoparasitoid Aphidius gifuensis.Scientific reports · 2017Article
- Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly.Scientific reports · 2017Article
- Review
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8 authors.
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Abstract
backgroundAedes albopictus is a globally invasive mosquito and a major vector of arboviruses, including dengue, Zika and Chikungunya. Olfactory-related behaviors, particularly host-seeking, offer opportunities to disrupt the disease-transmission process. A better understanding of odorant receptors (ORs) may assist in explaining host selection and location, and contribute to novel strategy of vector control.
methodsBased on previous prediction of 158 putative odorant receptors by Ae. albopictus genome analysis, 29 AalORs were selected for tissue-specific expression profiles in the present study. AalOrco (AalOR7), AalOR10 and AalOR88, highly expressed in female olfactory tissues, were chosen for further structure predictions as well as functional validation including calcium imaging assay in human embryonic kidney (HEK293) cells and RNA interference assay in Ae. albopictus. We also conducted electrophysiological and behavioral assays in mosquitoes after RNA interference of the three genes to determine their roles in host-seeking.
resultsThe results support previous conclusions that individual conventional (ORXs) and Orco can form heteromeric complexes to recognize odorants and respond to components of human volatiles in HEK293 cells. The reduction of AalOrco transcript levels led to a significant decrease in host-seeking and confusion in host preference. In contrast, AalOR10 and AalOR88 knockdown mosquitoes showed no significant behavioral differences compared with controls. The functions of conventional ORs at least AalOR10 and AalOR88 are abolished with inhibited expression of the Orco gene orthologs, along with the concomitant relevant olfactory behavior.
conclusionsCombining structural and functional data, we conclude that the product of the Orco gene in this mosquito is crucial for transmitting olfactory signaling and conventional ORs contribute directly to odorant recognition. Our results provide insight into the linkage between odorant receptors and host-seeking in this important vector species.
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