ArticlePlant cell reports2016
Rapid and efficient Agrobacterium-mediated transformation of sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Methylome and Transcriptome Analysis Reveals Differences in Callus Development and Plantlet Regeneration Capacity Between TwoPlants (Basel, Switzerland) · 2026Article
- Association mapping and identification of candidate genes for callus induction and regeneration using sorghum mature seeds.Frontiers in plant science · 2025Article
- R2R3 MYB transcription factor SbMYBHv33 negatively regulates sorghum biomass accumulation and salt tolerance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Current advances in the molecular regulation of abiotic stress tolerance in sorghumFrontiers in plant science · 2023Review
- Article
- Rapid and Highly Efficient Genetic Transformation and Application of Interleukin-17B Expressed in Duckweed as Mucosal Vaccine Adjuvant.Biomolecules · 2022Article
- Knock-down of phosphoenolpyruvate carboxylase 3 negatively impacts growth, productivity, and responses to salt stress in sorghum (Sorghum bicolor L.).The Plant journal : for cell and molecular biology · 2022Article
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- SbbHLH85, a bHLH member, modulates resilience to salt stress by regulating root hair growth in sorghum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Transcriptome sequencing analysis of sorghum callus with various regeneration capacities.Planta · 2021Article
- An efficient sorghum transformation system using embryogenic calli derived from mature seeds.PeerJ · 2021Article
- Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes.Plants (Basel, Switzerland) · 2020Article
- An Agrobacterium-delivered CRISPR/Cas9 system for targeted mutagenesis in sorghum.Plant biotechnology journal · 2020Article
- Progress in Optimization ofInternational journal of molecular sciences · 2018Review
- Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2.Plant cell reports · 2017Article
- Robust genetic transformation of sorghum (Plant methods · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA rapid and efficient Agrobacterium -mediated transformation system in sorghum has been developed employing standard binary vectors and bar gene as a selectable marker. Sorghum (Sorghum bicolor) is an important food and biofuel crop worldwide, for which improvements in genetic transformation are needed to study its biology and facilitate agronomic and commercial improvement. Here, we report optimization of regeneration and transformation of public sorghum genotype P898012 using standard binary vectors and bar gene as a selectable marker. The tissue culture regeneration time frame has been reduced to 7-12 weeks with a yield of over 18 plants per callus, and the optimized transformation system employing Agrobacterium tumefaciens strain AGL1 and the bar with a MAS promoter achieved an average frequency over 14 %. Of randomly analyzed independent transgenic events, 40-50 % carry single copy of integrated T-DNA. Some independent transgenic events were derived from the same embryogenic callus lines, but a 3:1 Mendelian segregation ratio was found in all transgenic events with single copy as estimated by Southern blots. The system described here should facilitate studies of sorghum biology and agronomic improvement.
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