Evidence map›Paper›PMID 27350036›Full record

ArticleStem cells (Dayton, Ohio)2016

Human Mesenchymal Stem Cell-Derived Microvesicles Prevent the Rupture of Intracranial Aneurysm in Part by Suppression of Mast Cell Activation via a PGE2-Dependent Mechanism.

Jia Liu, Atsushi Kuwabara, Yoshinobu Kamio, Shuling Hu, Jeonghyun Park, Tomoki Hashimoto, Jae-Woo Lee

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 2 syntheses or guidelines pooled it, 66 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jia LiuDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
Atsushi KuwabaraDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
Yoshinobu KamioDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
Shuling HuDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
Jeonghyun ParkDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
Tomoki HashimotoDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
Jae-Woo LeeDepartment of Anesthesia and Perioperative Care, University of California, San Francisco, California, USA.
University of California, San Francisco · US

Funding

Human Mesenchymal Stem Cell Microvesicles for the Treatment of Acute Lung InjuryR01HL113022 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LEE, JAE WOO · 2012 to 2020
$4.1M
Intracranial Aneurysm Pathogenesis-Roles of Vascular Remodeling and InflammationR01NS055876 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HASHIMOTO, TOMOKI · 2007 to 2015
$3.0M
The Role of Mast Cells in the Pathophysiology of Intracranial AneurysmR01NS082280 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HASHIMOTO, TOMOKI · 2013 to 2017
$1.7M
NHLBI NIH HHS R01 HL113022NINDS NIH HHS R01 NS055876NINDS NIH HHS R01 NS082280
6 · The paper itself

Abstract

Activation of mast cells participates in the chronic inflammation associated with cerebral arteries in intracranial aneurysm formation and rupture. Several studies have shown that the anti-inflammatory effect of mesenchymal stem cells (MSCs) is beneficial for the treatment of aneurysms. However, some long-term safety concerns exist regarding stem cell-based therapy for clinical use. We investigated the therapeutic potential of microvesicles (MVs) derived from human MSCs, anuclear membrane bound fragments with reparative properties, in preventing the rupture of intracranial aneurysm in mice, particularly in the effect of MVs on mast cell activation. Intracranial aneurysm was induced in C57BL/6 mice by the combination of systemic hypertension and intrathecal elastase injection. Intravenous administration of MSC-derived MVs on day 6 and day 9 after aneurysm induction significantly reduced the aneurysmal rupture rate, which was associated with reduced number of activated mast cells in the brain. A23187-induced activation of both primary cultures of murine mast cells and a human mast cell line, LAD2, was suppressed by MVs treatment, leading to a decrease in cytokine release and tryptase and chymase activities. Upregulation of prostaglandin E2 (PGE2) production and E-prostanoid 4 (EP4) receptor expression were also observed on mast cells with MVs treatment. Administration of an EP4 antagonist with the MVs eliminated the protective effect of MVs against the aneurysmal rupture in vivo. Human MSC-derived MVs prevented the rupture of intracranial aneurysm, in part due to their anti-inflammatory effect on mast cells, which was mediated by PGE2 production and EP4 activation. Stem Cells 2016;34:2943-2955.

Indexed as

Administration, IntravenousAneurysm, RupturedAnimalsAnti-Inflammatory AgentsBone Marrow CellsCalcimycinCell-Derived MicroparticlesDinoprostoneEndocytosisHumansImmunomodulationImmunosuppression TherapyIntracranial AneurysmMaleMast CellsMesenchymal Stem CellsAnti-Inflammatory AgentsCalcimycinDinoprostoneReceptors, Prostaglandin E, EP4 SubtypeRNA, MessengerIntracranial aneurysmMast cellsMesenchymal stem cellsMicrovesiclesProstaglandin E2

Identifiers

PMID27350036
PMCPMC5287411
OpenAlexW2461132302

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.