ArticleScientific reports2016
Guanine-rich sequences inhibit proofreading DNA polymerases.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Optimizing PCR amplification of GC-rich nicotinic acetylcholine receptor subunits from invertebrates.Biochemistry and biophysics reports · 2025Article
- Sweet enhancers of polymerase chain reaction.PloS one · 2024Article
- Maximum depth sequencing reveals an ON/OFF replication slippage switch and apparent in vivo selection for bifidobacterial pilus expression.Scientific reports · 2022Article
- Wristwatch PCR: A Versatile and Efficient Genome Walking Strategy.Frontiers in bioengineering and biotechnology · 2022Article
- Fourth Report on Chicken Genes and Chromosomes 2022.Cytogenetic and genome research · 2022Review
- Dual Deep Sequencing Improves the Accuracy of Low-Frequency Somatic Mutation Detection in Cancer Gene Panel Testing.International journal of molecular sciences · 2020Article
- A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells.Scientific reports · 2019Article
- An improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution.Scientific reports · 2019Article
- TT(N)mGCCTC inhibits archaeal family B DNA polymerases.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA polymerases with proofreading activity are important for accurate amplification of target DNA. Despite numerous efforts have been made to improve the proofreading DNA polymerases, they are more susceptible to be failed in PCR than non-proofreading DNA polymerases. Here we showed that proofreading DNA polymerases can be inhibited by certain primers. Further analysis showed that G-rich sequences such as GGGGG and GGGGHGG can cause PCR failure using proofreading DNA polymerases but not Taq DNA polymerase. The inhibitory effect of these G-rich sequences is caused by G-quadruplex and is dose dependent. G-rich inhibitory sequence-containing primers can be used in PCR at a lower concentration to amplify its target DNA fragment.
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