ArticleBiomaterials2016
N-terminal specific conjugation of extracellular matrix proteins to 2-pyridinecarboxaldehyde functionalized polyacrylamide hydrogels.
Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
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- Enhanced PDMS Functionalization for Organ-on-a-Chip Platforms Using Ozone and Sulfo-SANPAH: A Simple Approach for Biomimetic Long-Term Cell Cultures.Advanced healthcare materials · 2025Article
- Covalent Attachment of Functional Proteins to Microfiber Surfaces via a General Strategy for Site-Selective Tetrazine Ligation.ACS applied materials & interfaces · 2024Article
- Regulation of cell attachment, spreading, and migration by hydrogel substrates with independently tunable mesh size.Acta biomaterialia · 2022Article
- Strategy for Conjugating Oligopeptides to Mesoporous Silica Nanoparticles Using Diazirine-Based Heterobifunctional Linkers.Nanomaterials (Basel, Switzerland) · 2022Article
- StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells.Nature communications · 2021Article
- Measuring expression heterogeneity of single-cell cytoskeletal protein complexes.Nature communications · 2021Article
- Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell-ECM Interactions.ACS biomaterials science & engineering · 2021Article
- Substrate Resistance to Traction Forces Controls Fibroblast Polarization.Biophysical journal · 2020Article
- Cofilin is required for polarization of tension in stress fiber networks during migration.Journal of cell science · 2020Article
- Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
- Longer collagen fibers trigger multicellular streaming on soft substrates via enhanced forces and cell-cell cooperation.Journal of cell science · 2019Article
- Extracellular matrix type modulates mechanotransduction of stem cells.Acta biomaterialia · 2019Article
- Extracellular Matrix Geometry and Initial Adhesive Position Determine Stress Fiber Network Organization during Cell Spreading.Cell reports · 2019Article
- Hydrogels with enhanced protein conjugation efficiency reveal stiffness-induced YAP localization in stem cells depends on biochemical cues.Biomaterials · 2019Article
- Biochemical Ligand Density Regulates Yes-Associated Protein Translocation in Stem Cells through Cytoskeletal Tension and Integrins.ACS applied materials & interfaces · 2019Article
- From Synthesis to Characterization of Site-Selective PEGylated Proteins.Frontiers in pharmacology · 2019Review
- Achieving Controlled Biomolecule-Biomaterial Conjugation.Chemical reviews · 2018Review
- Actomyosin stress fiber subtypes have unique viscoelastic properties and roles in tension generation.Molecular biology of the cell · 2018Article
- Activation of ROCK and MLCK tunes regional stress fiber formation and mechanics via preferential myosin light chain phosphorylation.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Polyacrylamide hydrogels have been used extensively to study cell responses to the mechanical and biochemical properties of extracellular matrix substrates. A key step in fabricating these substrates is the conjugation of cell adhesion proteins to the polyacrylamide surfaces, which typically involves nonspecifically anchoring these proteins via side-chain functional groups. This can result in a loss of presentation control and altered bioactivity. Here, we describe a new functionalization strategy in which we anchor full-length extracellular matrix proteins to polyacrylamide substrates using 2-pyridinecarboxaldehyde, which can be co-polymerized into polyacrylamide gels and used to immobilize proteins by their N-termini. This one-step reaction proceeds under mild aqueous conditions and does not require additional reagents. We demonstrate that these substrates can readily conjugate to various extracellular matrix proteins, as well as promote cell adhesion and spreading. Notably, this chemistry supports the assembly and cellular remodeling of large collagen fibers, which is not observed using conventional side-chain amine-conjugation chemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.