ArticlePloS one2016
Dimethyl Sulfoxide Perturbs Cell Cycle Progression and Spindle Organization in Porcine Meiotic Oocytes.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Research Progress on Mammalian Oocyte Vitrification: From Damage Mechanisms to Optimization Strategies.Animals : an open access journal from MDPI · 2026Review
- Optimization of mouse oocyte vitrification through microencapsulation in sodium alginate hydrogel.Journal of assisted reproduction and genetics · 2025Article
- A comparison of calcification mechanisms in haploid and diploid cells of the coccolithophore Calcidiscus leptoporus (Murray & Blackman 1898).Journal of phycology · 2025Article
- Oocytes Vitrification Using Automated Equipment Based on Microfluidic Chip.Annals of biomedical engineering · 2025Article
- The role of cannabinoid agonists and antagonists on folliculogenesis and evolutionary events in the mouse ovary.Iranian journal of basic medical sciences · 2025Article
- The effect of DMSO on Saccharomyces cerevisiae yeast with different energy metabolism and antioxidant status.Scientific reports · 2024Article
- Article
- The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos.Scientific reports · 2023Article
- Efficacy of soluble lansoprazole-impregnated beta-tricalcium phosphate for bone regeneration.Scientific reports · 2022Article
- DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development.Porcine health management · 2021Article
- Effects of RAD51-stimulatory compound 1 (RS-1) and its vehicle, DMSO, on pig embryo culture.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- Mouse oocyte vitrification with and without dimethyl sulfoxide: influence on cryo-survival, development, and maternal imprinted gene expression.Journal of assisted reproduction and genetics · 2021Article
- Bioinformatics Analysis Makes Revelation to Potential Properties on Regulation and Functions of Human Sox2.Pathology oncology research : POR · 2020Article
- Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs).Stem cells international · 2020Article
- DMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro.Scientific reports · 2019Article
- Heat shock protein 90α couples with the MAPK-signaling pathway to determine meiotic maturation of porcine oocytes.Journal of animal science · 2018Article
- Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes.Scientific reports · 2018Article
- Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation.The Journal of biological chemistry · 2018Article
- DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes.Scientific reports · 2017Article
- Toxic effects of dimethyl sulfoxide on red blood cells, platelets, and vascular endothelial cellsFEBS open bio · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiotic maturation of mammalian oocytes is a precisely orchestrated and complex process. Dimethyl sulfoxide (DMSO), a widely used solvent, drug, and cryoprotectant, is capable of disturbing asymmetric cytokinesis of oocyte meiosis in mice. However, in pigs, DMSO's effect on oocyte meiosis still remains unknown. We aimed to evaluate if DMSO treatment will affect porcine oocyte meiosis and the underlying molecular changes as well. Interestingly, we did not observe the formation of the large first polar body and symmetric division for porcine oocytes treated with DMSO, contrary to findings reported in mice. 3% DMSO treatment could inhibit cumulus expansion, increase nuclear abnormality, disturb spindle organization, decrease reactive oxygen species level, and elevate mitochondrial membrane potential of porcine oocytes. There was no effect on germinal vesicle breakdown rate regardless of DMSO concentration. 3% DMSO treatment did not affect expression of genes involved in spindle organization (Bub1 and Mad2) and apoptosis (NF-κB, Pten, Bcl2, Caspase3 and Caspase9), however, it significantly decreased expression levels of pluripotency genes (Oct4, Sox2 and Lin28) in mature oocytes. Therefore, we demonstrated that disturbed cumulus expansion, chromosome alignment, spindle organization and pluripotency gene expression could be responsible for DMSO-induced porcine oocyte meiotic arrest and the lower capacity of subsequent embryo development. Our results provide new insights on DMSO's effect on porcine oocyte meiosis and raise safety concerns over DMSO's usage on female reproduction in both farm animals and humans.
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