ArticleInternational journal of molecular sciences2016
Extraction, Characterization and Immunological Activity of Polysaccharides from Rhizoma gastrodiae.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- A Comparison Study on Polysaccharides Extracted fromPolymers · 2025Article
- The Chemical Profiling and Immunological Activity of Polysaccharides from the Rhizome ofMolecules (Basel, Switzerland) · 2025Article
- Extraction, Purification, Structural Characteristics, Biological Activities, and Applications of Polysaccharides fromMolecules (Basel, Switzerland) · 2025Review
- From molecules to medicine: a systematic review of Gastrodia elata's bioactive metabolites and therapeutic potential.Frontiers in pharmacology · 2025Review
- Research progress ofFrontiers in chemistry · 2024Review
- Comparative analysis of antioxidant activity and structural changes ofFrontiers in nutrition · 2024Article
- Diversity ofFrontiers in microbiology · 2024Article
- Extraction, Purification, and Structural Characterization of Polysaccharides fromMolecules (Basel, Switzerland) · 2023Article
- Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights ofMolecules (Basel, Switzerland) · 2023Article
- Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated fromRSC advances · 2022Article
- Purification, structural characterization and immunological activity ofFrontiers in nutrition · 2022Article
- Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties ofFrontiers in chemistry · 2022Article
- Optimization of trypsin extraction technology of Allium cepa L. polysaccharide by response surface methodology and the antitumor effects through immunomodulation.Bioengineered · 2021Article
- Structural Characterization of a Polysaccharide fromMolecules (Basel, Switzerland) · 2021Article
- Article
- Article
- Review
- Article
- Medicinal Plants and Isolated Molecules Demonstrating Immunomodulation Activity as Potential Alternative Therapies for Viral Diseases Including COVID-19.Frontiers in immunologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP) extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b) were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 μg/mL) and LPS (2 μg/mL) had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05). All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.