ArticleMolecules (Basel, Switzerland)2016
A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues.
Article in Molecules (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Fungal cellulases: a comprehensive review on production, innovations, and applications.Archives of microbiology · 2026Review
- Mechanistic insight into the anti-alternaria activity of bimetallic zinc oxide and silver/zinc oxide nanoparticles.Heliyon · 2024Article
- Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion.Microorganisms · 2023Review
- Prospecting Microbial Genomes for Biomolecules and Their Applications.Indian journal of microbiology · 2022Review
- Simultaneous Enhancement of Thermostability and Catalytic Activity of a Metagenome-Derived β-Glucosidase Using Directed Evolution for the Biosynthesis of Butyl Glucoside.International journal of molecular sciences · 2019Article
- Genomic, Transcriptomic and Enzymatic Insight into Lignocellulolytic System of a Plant PathogenBiomolecules · 2019Article
- Biochemical Characterization of a Novel Monospecific Endo-β-1,4-Glucanase Belonging to GH Family 5 From a Rhizosphere Metagenomic Library.Frontiers in microbiology · 2019Article
- New Bacterial Phytase through Metagenomic Prospection.Molecules (Basel, Switzerland) · 2018Article
- A Comparative Review on Microbiota Manipulation: Lessons From Fish, Plants, Livestock, and Human Research.Frontiers in nutrition · 2018Review
- Identification and molecular characterization of a metagenome-derived L-lysine decarboxylase gene from subtropical soil microorganisms.PloS one · 2017Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many naturally-occurring cellulolytic microorganisms are not readily cultivable, demanding a culture-independent approach in order to study their cellulolytic genes. Metagenomics involves the isolation of DNA from environmental sources and can be used to identify enzymes with biotechnological potential from uncultured microbes. In this study, a gene encoding an endoglucanase was cloned from red rice crop residues using a metagenomic strategy. The amino acid identity between this gene and its closest published counterparts is lower than 70%. The endoglucanase was named EglaRR01 and was biochemically characterized. This recombinant protein showed activity on carboxymethylcellulose, indicating that EglaRR01 is an endoactive lytic enzyme. The enzymatic activity was optimal at a pH of 6.8 and at a temperature of 30 °C. Ethanol production from this recombinant enzyme was also analyzed on EglaRR01 crop residues, and resulted in conversion of cellulose from red rice into simple sugars which were further fermented by Saccharomyces cerevisiae to produce ethanol after seven days. Ethanol yield in this study was approximately 8 g/L. The gene found herein shows strong potential for use in ethanol production from cellulosic biomass (second generation ethanol).
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