ArticleNature biotechnology2016
Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.
Article in Nature biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 179 papers.
What it found
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Who cites it
179 citing papers in PubMed.
- Large cranial windows distort auditory cortical maps and degrade feature integration in secondary auditory cortex.iScience · 2026Article
- Longitudinal Awake Mouse Brain Imaging Using Functional Ultrasound and Functional Ultrasound Localization Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) for multiparameter and quantitative brain function imaging.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Probing inter-areal computations with a two-photon holographic mesoscope.Nature neuroscience · 2026Article
- Stars2Cells: Astrometric Tracking of Neurons Across Imaging Sessions.bioRxiv : the preprint server for biology · 2026Article
- CortexCAM: A Camera Array Microscope for Cortex-Wide Cellular Imaging in Freely Locomoting Mice.Research square · 2026Article
- STIMscope: centimeter-scale all-optical imaging and patterned optogenetic manipulation at single-cell resolution.bioRxiv : the preprint server for biology · 2026Article
- Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging.Light, science & applications · 2026Article
- Long-range axon branching: contributions to brain network plasticity and repair.Nature reviews. Neuroscience · 2026Review
- Mesoscale Recovery of Microglial and Neuronal Dynamics After Craniotomy Across Wide Cortex in Transgenic Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- Large cranial windows distort sensory maps and degrade feature integration in higher-order cortex.bioRxiv : the preprint server for biology · 2026Article
- Cortex-Wide Cellular Imaging in Freely Locomoting Mice Using Cortex Camera Array Microscope (CortexCAM).bioRxiv : the preprint server for biology · 2026Article
- Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise.Neurophotonics · 2026Article
- Cellular multipoint adaptive technology for two-photon mesoscope.Neurophotonics · 2026Article
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Pan-cortical cellular imaging in freely behaving mice using a miniaturized micro-camera array microscope (mini-MCAM).Science advances · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
119 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.