ArticleInternational journal of ophthalmology & eye science2016
High Glucose and Glucose Deprivation Modulate Müller Cell Viability and VEGF Secretion.
Article in International journal of ophthalmology & eye science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 12 citations in OpenAlex.
- Beyond Glucose: Palmitic Acid Influences VEGFA-VEGFR2 Angiogenic Signaling in Müller Glial Cells.International journal of molecular sciences · 2026Article
- Neuroprotective effects ofFrontiers in molecular biosciences · 2026Article
- Suppression of cAMP/PKA/CREB signaling ameliorates retinal injury in diabetic retinopathy.The Kaohsiung journal of medical sciences · 2023Article
- High glucose induces an early and transient cytoprotective autophagy in retinal Müller cells.Endocrine · 2022Article
- Biochemical mechanism underlying the pathogenesis of diabetic retinopathy and other diabetic complications in humans: the methanol-formaldehyde-formic acid hypothesis.Acta biochimica et biophysica Sinica · 2022Article
- Redox and Calcium Alterations of a Müller Cell Line Exposed to Diabetic Retinopathy-Like Environment.Frontiers in cellular neuroscience · 2022Article
- Starvation to Glucose Reprograms Development of Neurovascular Unit in Embryonic Retinal Cells.Frontiers in cell and developmental biology · 2021Article
- Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells.Journal of ophthalmology · 2019Article
- Palmitic Acid Induces Müller Cell Inflammation that is Potentiated by Co-treatment with Glucose.Scientific reports · 2018Article
- Effect of Glucose on Retinal Endothelial Cell Viability and VEGF Secretion.HSOA journal of cell biology & cell metabolism · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeDiabetic retinopathy is manifested by excessive angiogenesis and high level of vascular endothelial growth factor (VEGF) in the eye.
methodsHuman (MIO-M1) and rat (rMC-1) Müller cells were treated with 0, 5.5, or 30mM glucose for 24 hours. Viable cell counts were obtained by Trypan Blue Dye Exclusion Method. ELISA was used to determine VEGF levels in cell medium.
resultsCompared to 24 hour treatment by 5.5mM glucose, MIO-M1 and rMC-1 in 30mM glucose increased in viable cell number by 38% and 24% respectively. In contrast, viable cells in 0mM glucose decreased by 28% and 50% respectively. Compared to 5.5mM, MIO-M1 and rMC-1 in 30mM glucose had increased levels of VEGF in cell medium (pg/ml by 24% and 20%) and also VEGF concentration in cells held in 0mM increased by 47% and 10% respectively. In both MIO-M1 and rMC-1, the amount of VEGF secreted per cell increased by about 100% when glucose was changed from 5.5 to 0mM but decreased slightly (17% in MIO-M1 and 11% in rMC-1) when glucose was increased from 5.5 to 30mM.
conclusionsOur results show that MIO-M1 and rMC-1 are highly responsive to changes in glucose concentrations. 30mM compared to 5.5mM significantly increased cell viability but induced a significant change in VEGF secretion per cell in rMC-1 only. At 0, 5.5, and 30mM glucose, MIO-M1 secreted about 5-7-fold higher level of VEGF (pg/cell) than rMC-1. The mechanism of glucose-induced changes in rMC-1 and MIO-M1 cell viability and VEGF secretion remains to be elucidated.
Indexed as
Identifiers
27347496PMC4917289W2318278118What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.