Evidence map›Paper›PMID 27347456›Full record

ArticleApplications in plant sciences2016

The report of my death was an exaggeration: A review for researchers using microsatellites in the 21st century.

Richard G J Hodel, M Claudia Segovia-Salcedo, Jacob B Landis, Andrew A Crowl, Miao Sun, Xiaoxian Liu, Matthew A Gitzendanner, Norman A Douglas, Charlotte C Germain-Aubrey, Shichao Chen and 2 more

Open access · goldAbstract read
In one paragraph

Article in Applications in plant sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed
20.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 215 citations in OpenAlex.

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  14. International journal of molecular sciences · 2024
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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Richard G J HodelDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA; Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA.
M Claudia Segovia-SalcedoDepartamento de Ciencias de la Vida, Universidad de las Fuerzas Armadas-ESPE, Sangolqui, Ecuador.
Jacob B LandisDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA; Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA.
Andrew A CrowlDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA; Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA.
Miao SunFlorida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA.
Xiaoxian LiuDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA; Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA.
Matthew A GitzendannerDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA.
Norman A DouglasDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA.
Charlotte C Germain-AubreyFlorida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA.
Shichao ChenCollege of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Douglas E SoltisDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA; Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA; The Genetics Institute, University of Florida, Gainesville, Florida 32611 USA.
Pamela S SoltisFlorida Museum of Natural History, University of Florida, Gainesville, Florida 32611 USA; The Genetics Institute, University of Florida, Gainesville, Florida 32611 USA.
Florida Museum of Natural History · USUniversity of Florida · USTongji University · CNUniversidad de las Fuerzas Armadas ESPE · EC

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microsatellites, or simple sequence repeats (SSRs), have long played a major role in genetic studies due to their typically high polymorphism. They have diverse applications, including genome mapping, forensics, ascertaining parentage, population and conservation genetics, identification of the parentage of polyploids, and phylogeography. We compare SSRs and newer methods, such as genotyping by sequencing (GBS) and restriction site associated DNA sequencing (RAD-Seq), and offer recommendations for researchers considering which genetic markers to use. We also review the variety of techniques currently used for identifying microsatellite loci and developing primers, with a particular focus on those that make use of next-generation sequencing (NGS). Additionally, we review software for microsatellite development and report on an experiment to assess the utility of currently available software for SSR development. Finally, we discuss the future of microsatellites and make recommendations for researchers preparing to use microsatellites. We argue that microsatellites still have an important place in the genomic age as they remain effective and cost-efficient markers.

Indexed as

genotyping by sequencing (GBS)microsatellite developmentnext-generation sequencing (NGS)restriction site associated DNA sequencing (RAD-Seq)simple sequence repeats (SSR)transcriptomes

Identifiers

PMID27347456
PMCPMC4915923
OpenAlexW2439694667

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.