Evidence map›Paper›PMID 27347452›Full record

ArticleApplications in plant sciences2016

Development of microsatellite markers for Fargesia denudata (Poaceae), the staple-food bamboo of the giant panda.

Yan Lv, Tao Yu, Sihai Lu, Cheng Tian, Junqing Li, Fang K Du

Abstract read
In one paragraph

Article in Applications in plant sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yan LvCollege of Forestry, Key Laboratory for Forest Resources and Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, People's Republic of China.
Tao YuCollege of Forestry, Key Laboratory for Forest Resources and Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, People's Republic of China.
Sihai LuCollege of Life Science, Lanzhou University, Lanzhou 730000, Gansu, People's Republic of China.
Cheng TianCollege of Forestry, Key Laboratory for Forest Resources and Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, People's Republic of China.
Junqing LiCollege of Forestry, Key Laboratory for Forest Resources and Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, People's Republic of China.
Fang K DuCollege of Forestry, Key Laboratory for Forest Resources and Ecosystem Processes of Beijing, Beijing Forestry University, Beijing 100083, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyThere is a need for microsatellite primers to analyze genetic parameters of Fargesia denudata (Poaceae), the staple-food bamboo of the giant panda (Ailuropoda melanoleuca). METHODS AND

resultsUsing next-generation sequencing technology, we obtained a 75-Mb assembled sequence of F. denudata and identified 182 microsatellites. Primer pairs for 70 candidate microsatellite markers were selected and validated in four individuals, and 42 primer pairs generated reliable amplicons. Fourteen of 16 tested markers were found to be polymorphic in 72 individuals from four F. denudata populations. The number of alleles ranged from two to 19 per locus; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.87, respectively. The transferability of these 16 novel microsatellite markers was validated in five related species.

conclusionsThese markers will be useful for examining the genetic diversity, genetic structure, and cloning of F. denudata, the staple-food bamboo of the giant panda, and related bamboo species.

Indexed as

Fargesia denudatagiant pandamicrosatellitesnext-generation sequencingPoaceae

Identifiers

PMID27347452
PMCPMC4915919

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