ArticleStem cell reports2016
Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes.
Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 79 citations in OpenAlex.
- Long-Term Labeling and Differentiation Monitoring of Mouse Spermatogonial Stem Cells Using CellREADR.Biotech (Basel (Switzerland)) · 2026Article
- lncRNA-operated stem cell fate control: RPS3 mediates the in situ Gm16751-Zhx3 interactome to program spermatogonial stem cell differentiation.Cell & bioscience · 2026Article
- The influence and mechanism of gut microbiota on spermatogenesis.Cell biology and toxicology · 2026Review
- Review
- SPRY4-dependent ERK negative feedback demarcates functional adult stem cells in the male mouse germline†.Biology of reproduction · 2023Article
- Evidence for RA-dependent meiosis onset in a turtle embryo.Cell and tissue research · 2023Article
- Targeting the retinoic acid signaling pathway as a modern precision therapy against cancers.Frontiers in cell and developmental biology · 2023Review
- Review
- scATAC-Seq reveals heterogeneity associated with spermatogonial differentiation in cultured male germline stem cells.Scientific reports · 2022Article
- Retinoic acid can improve autophagy through depression of the PI3K-Akt-mTOR signaling pathway via RARα to restore spermatogenesis in cryptorchid infertile rats.Genes & diseases · 2022Article
- Investigation of Signals and Transcription Factors for The Generation of Female Germ-Like Cells.Cell journal · 2022Article
- MicroRNA-202 safeguards meiotic progression by preventing premature SEPARASE-mediated REC8 cleavage.EMBO reports · 2022Article
- Establishment of aBiology · 2022Article
- AOP Key Event Relationship report: Linking decreased retinoic acid levels with disrupted meiosis in developing oocytes.Current research in toxicology · 2022Article
- Direct reprogramming of human Sertoli cells into male germline stem cells with the self-renewal and differentiation potentials via overexpressing DAZL/DAZ2/BOULE genes.Stem cell reports · 2021Article
- Characterization of Breed Specific Differences in Spermatozoal Transcriptomes of Sheep in Australia.Genes · 2021Article
- PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by RegulatingFrontiers in cell and developmental biology · 2021Article
- Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation.Frontiers in physiology · 2021Article
- In Vitro Meiosis of Male Germline Stem Cells.Stem cell reports · 2020Article
- Analysis of lncRNA Expression Profile during the Formation of Male Germ Cells in Chickens.Animals : an open access journal from MDPI · 2020Article
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulated by RA were identified by RNA sequencing. RA in combination with pup Sertoli cell co-culture resulted in a higher induction efficiency of 28%. Comparisons in the transcriptomic profiles of the induced spermatogenic cells and the isolated ones revealed the progressive induction of the germ cells. Using this model, we showed that Stra8, Agpat3, Fam57a, Wdr91, and Sox30 contributed to the proliferation and meiosis initiation differentially. In conclusion, we have efficiently generated spermatocytes using an RA/pup Sertoli cell-based in vitro model and provided proof-of-concept evidence for its application in identifying genes involved in mammalian meiosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.