ArticleJournal of neuroscience methods2016
Micro-computed tomography in murine models of cerebral cavernous malformations as a paradigm for brain disease.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Rapamycin Partially Reverts Cavernoma Endothelial Cell Phenotype and, When Combined With Lapatinib, Ameliorates Chronic Lesions.Journal of cellular and molecular medicine · 2026Article
- TIE2 links MEKK3-KLF2/4 and PI3K signaling in cerebral cavernous malformation.The Journal of experimental medicine · 2026Article
- High-resolution imaging and three-dimensional model of the feline spinal cord.Frontiers in neuroanatomy · 2026Article
- A mouse model of stereotactic radiosurgery-induced neuroinflammation and blood-brain barrier compromise.Acta neuropathologica communications · 2025Article
- Except for Robust Outliers, Rapamycin Increases Lesion Burden in a Murine Model of Cerebral Cavernous Malformations.Translational stroke research · 2025Article
- Mild Hypoxia Accelerates Cerebral Cavernous Malformation Disease Through CX3CR1-CX3CL1 Signaling.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- mTORC1 Inhibitor Rapamycin Inhibits Growth of Cerebral Cavernous Malformation in Adult Mice.Stroke · 2023Article
- High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models.Scientific reports · 2022Article
- Astrocytes propel neurovascular dysfunction during cerebral cavernous malformation lesion formation.The Journal of clinical investigation · 2021Article
- Cerebral Cavernous Malformation: From Mechanism to Therapy.Circulation research · 2021Review
- Article
- Cerebral cavernous malformations are driven by ADAMTS5 proteolysis of versican.The Journal of experimental medicine · 2020Article
- NIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice.Biomaterials science · 2020Article
- A Brain-Targeted Orally Available ROCK2 Inhibitor Benefits Mild and Aggressive Cavernous Angioma Disease.Translational stroke research · 2020Article
- Distinct cellular roles for PDCD10 define a gut-brain axis in cerebral cavernous malformation.Science translational medicine · 2019Article
- Cavernous angiomas: deconstructing a neurosurgical disease.Journal of neurosurgery · 2019Review
- Phenotypic characterization of murine models of cerebral cavernous malformations.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Rho Kinase Inhibition Blunts Lesion Development and Hemorrhage in Murine Models of Aggressive Pdcd10/Ccm3 Disease.Stroke · 2019Article
- Investigating the microstructure of plant leaves in 3D with lab-based X-ray computed tomography.Plant methods · 2018Article
- Thrombospondin1 (TSP1) replacement prevents cerebral cavernous malformations.The Journal of experimental medicine · 2017Article
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Authors and funding
20 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundCerebral cavernous malformations (CCMs) are hemorrhagic brain lesions, where murine models allow major mechanistic discoveries, ushering genetic manipulations and preclinical assessment of therapies. Histology for lesion counting and morphometry is essential yet tedious and time consuming. We herein describe the application and validations of X-ray micro-computed tomography (micro-CT), a non-destructive technique allowing three-dimensional CCM lesion count and volumetric measurements, in transgenic murine brains. NEW
methodWe hereby describe a new contrast soaking technique not previously applied to murine models of CCM disease. Volumetric segmentation and image processing paradigm allowed for histologic correlations and quantitative validations not previously reported with the micro-CT technique in brain vascular disease.
resultsTwenty-two hyper-dense areas on micro-CT images, identified as CCM lesions, were matched by histology. The inter-rater reliability analysis showed strong consistency in the CCM lesion identification and staging (K=0.89, p<0.0001) between the two techniques. Micro-CT revealed a 29% greater CCM lesion detection efficiency, and 80% improved time efficiency. COMPARISON WITH EXISTING
methodSerial integrated lesional area by histology showed a strong positive correlation with micro-CT estimated volume (r(2)=0.84, p<0.0001).
conclusionsMicro-CT allows high throughput assessment of lesion count and volume in pre-clinical murine models of CCM. This approach complements histology with improved accuracy and efficiency, and can be applied for lesion burden assessment in other brain diseases.
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