ArticleCell2016
Sugar Synthesis from CO2 in Escherichia coli.
Article in Cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
129 citing papers in PubMed, 379 citations in OpenAlex.
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- NAViFluX: a visualization‑centric platform for interactive analysis, refinement and design of genome‑scale metabolic networks.Bioinformatics (Oxford, England) · 2026Article
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- An Efficient COAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Water Vapor-Enhanced Selective Production of Methane During Photothermal COAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway.Journal of biological engineering · 2025Article
- Metabolic Engineering of Yeasts: A Key Cell Factory Platform for Advanced Biomanufacturing.Journal of fungi (Basel, Switzerland) · 2025Article
- Fructose-1,6-bisphosphatase is involved in heterotrophic growth and glycogen metabolism in cyanobacteria.Journal of experimental botany · 2025Article
- A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity.Microbiology (Reading, England) · 2025Review
- A blueprint for designing the next-generation of synthetic CNature communications · 2025Review
- Considering Metabolic Context in Enzyme Evolution and Design.Biochemistry · 2025Review
- Mixotrophy for carbon-conserving waste upcycling.PLoS computational biology · 2025Article
- Energetic constraints of metal-reducing bacteria as biocatalysts for microbial electrosynthesis.Biotechnology for biofuels and bioproducts · 2025Article
- Carbon sequestration pathways in microorganisms: Advances, strategies, and applications.Engineering microbiology · 2025Review
- Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection.Nature communications · 2025Article
- Bringing carbon to life via one-carbon metabolism.Trends in biotechnology · 2025Review
- Article
69 more citing papers are in PubMed but not listed here.
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Authors and funding
19 authors at 2 institutions in 1 country.
Funding
Abstract
Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomass constituents by a fully functional Calvin-Benson-Bassham (CBB) cycle in E. coli. In the evolved bacteria, carbon fixation is performed via a non-native CBB cycle, while reducing power and energy are obtained by oxidizing a supplied organic compound (e.g., pyruvate). Genome sequencing reveals that mutations in flux branchpoints, connecting the non-native CBB cycle to biosynthetic pathways, are essential for this phenotype. The successful evolution of a non-native carbon fixation pathway, though not yet resulting in net carbon gain, strikingly demonstrates the capacity for rapid trophic-mode evolution of metabolism applicable to biotechnology. PAPERCLIP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.