Evidence map›Paper›PMID 27345005›Full record

ArticleAsian journal of andrology

Therapeutic effects of adipose-derived stem cells-based microtissues on erectile dysfunction in streptozotocin-induced diabetic rats.

Feng Zhou, Yu Hui, Hua Xin, Yong-De Xu, Hong-En Lei, Bi-Cheng Yang, Rui-Li Guan, Meng Li, Jian-Quan Hou, Zhong-Cheng Xin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian journal of andrology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Feng ZhouMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Yu HuiMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Hua XinDepartment of Ophthalmology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, China.
Yong-De XuMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Hong-En LeiMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Bi-Cheng YangMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Rui-Li GuanMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Meng LiDepartment of Urology, General Hospital of Ningxia Medical University, Ningxia Medical University, Ningxia 750021, China.
Jian-Quan HouDepartment of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.
Zhong-Cheng XinMolecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Peking University First Hospital · CNSoochow University · CNCapital Medical University · CNNingxia Medical University · CNPeking University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to explore the therapeutic effects of adipose-derived stem cells (ADSCs)-based microtissues (MTs) on erectile dysfunction (ED) in streptozotocin (STZ)-induced diabetic rats. Fifty-six 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ (60 mg kg-1 ), and 8 weeks later, the determined diabetic rats randomly received intracavernous (IC) injection of phosphate buffer solution (PBS), ADSCs, or MTs. Another eight normal rats equally got IC injection of PBS. MTs were generated with a hanging drop method, and the injected cells were tracked in ADSC- and MT-injected rats. Four weeks after the treatments, intracavernous pressure (ICP), histopathological changes in corpus cavernosum (CC), and functional proteins were measured. Rat cytokine antibody array was used to detect ADSCs or MTs lysate. The results showed that MTs expressed vascular endothelial growth factor (VEGF), nerve growth factor (NGF), and tumor necrosis factor-stimulated gene-6 (TSG-6). MTs injection had a higher retention than ADSCs injection and MTs treatment improved ICP, neuronal nitric oxide synthase (nNOS) expression, smooth muscle, and endothelial contents in diabetic rats, ameliorated local inflammation in CC better. Thus, our findings demonstrate that IC injection of MTs improves erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.

Indexed as

Penile ErectionStem Cell TransplantationActinsAdipose TissueAnimalsBlotting, WesternCell Adhesion MoleculesDiabetes Mellitus, ExperimentalDisease Models, AnimalErectile DysfunctionFluorescent Antibody TechniqueMaleNerve Growth FactorNF-kappa BNitric Oxide Synthase Type IPenisActa2 protein, ratActinsCell Adhesion MoleculesNerve Growth FactorNF-kappa BNitric Oxide Synthase Type ITnfaip6 protein, ratVascular Endothelial Growth Factor Avascular endothelial growth factor A, rat

Identifiers

PMID27345005
PMCPMC5227681
OpenAlexW2398827757

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.